Smooth Muscle Myosin II antibody

The structure of myosin II is fundamentally the same in all muscle types and consists of two heavy chains, each bound by 2 distinct, essential and regulatory light chains. The globular motor domain located at the N-terminal domain of the heavy chains, catalyzes ATP hydrolysis and interacts with actin, whilst the heptad repeat sequences of the heavy chain tail domains promote dimerization, forming an alpha-helical coiled coil at the C-terminal domain.
Rabbit anti Bovine Smooth Muscle Myosin II antibody does not cross-react with myosin of skeletal or non-muscle origin and can be used to specifically stain cultured smooth muscle cells and identify vascular and other smooth muscle types in tissue sections.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Vertebrates Expected from Sequence Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine smooth muscle myosin II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.006% Sodium Azide (NaN3) - Immunogen
- Bovine tracheal smooth muscle myosin.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Smooth Muscle Myosin II antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | |||
Immunohistology - Frozen | |||
Western Blotting | 1/150 |
- Western Blotting
- Rabbit anti Bovine smooth muscle myosin II antibody recognizes a band of approximately 200 kDa in western blotting analysis of differentiating smooth muscle cell culture lysates (Margariti et al. 2009).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Smooth Muscle Myosin II antibody
-
Xiao, Q. et al. (2011) Chromobox protein homolog 3 is essential for stem cell differentiation to smooth muscles in vitro and in embryonic arteriogenesis.
Arterioscler Thromb Vasc Biol. 31: 1842-52. -
Zhang, L. et al. (2010) Sp1-dependent activation of HDAC7 is required for platelet-derived growth factor-BB-induced smooth muscle cell differentiation from stem cells.
J Biol Chem. 285 (49): 38463-72. -
Margariti, A. et al. (2009) Splicing of HDAC7 modulates the SRF-myocardin complex during stem-cell differentiation towards smooth muscle cells.
J Cell Sci. 122: 460-70. -
Xiao, Q. et al. (2009) Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2.
Am J Physiol Cell Physiol. 296: C711-23. -
Wang, G. et al. (2012) Functional impact of heterogeneous nuclear ribonucleoprotein A2/B1 in smooth muscle differentiation from stem cells and embryonic arteriogenesis.
J Biol Chem. 287 (4): 2896-906. -
Zheng, X. et al. (2013) Angiotensin II promotes differentiation of mouse embryonic stem cells to smooth muscle cells through PI3-kinase signaling pathway and NF-κB.
Differentiation. 85: 41-54. -
Jin, M. et al. (2016) MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1.
Stem Cell Res. 17 (2): 277-284. -
Yu, X. et al. (2015) Upregulated sirtuin 1 by miRNA-34a is required for smooth muscle cell differentiation from pluripotent stem cells.
Cell Death Differ. 22 (7): 1170-80.
Further Reading
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Rosenfeld, S.S. et al. (1998) Structural and kinetic studies of phosphorylation-dependent regulation in smooth muscle myosin.
J Biol Chem. 273 (44): 28682-90.