S100 antibody

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Rabbit anti S100 Protein

Rabbit anti S100 Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP385HT C P 1 ml
AHP385 C P 1 ml
Rabbit anti S100 Protein antibody reacts with human S-100 A and S-100 B. S100 is present in brain glial cells and in ependymal cells. Rabbit anti S100 Protein antibody also labels Schwann cells of the peripheral nervous system.

Among tumours of the human central and peripheral nervous systems, schwannomas, ependymomas and astrogliomas of different stages, including glioblastomas show positive reactions. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin and in Langerhans cells in the skin.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
Rat
Western grey kangaroo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid, ready to use
Product Form
Serum - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Bovine brain S-100.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of S100 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat
Immunohistology - Paraffin Neat
Immunohistology - Frozen 1/10 1/40
Immunohistology - Paraffin 1/10 1/40
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Brain, skin or human melanoma
Histology Positive Control Tissue
Brain, skin or human melanoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for S100 antibody

  1. Kindblom, L.G. et al. (1984) S-100 protein in melanocytic tumors. An immunohistochemical investigation of benign and malignant melanocytic tumors and metastases of malignant melanoma and a characterization of the antigen in comparison to human brain.
    Acta Pathol Microbiol Immunol Scand A. 92 (4): 219-30.
  2. Lauriola, L. et al. (1984) Detection of S-100 labelled cells in nasopharyngeal carcinoma.
    J Clin Pathol. 37 (11): 1235-8.
  3. Lam, T.T. et al. (2003) Early glial responses after acute elevated intraocular pressure in rats.
    Invest Ophthalmol Vis Sci. 44 (2): 638-45.
  4. Etherington S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
    J Zool. Jun 26 [Epub ahead of print]

Further Reading

  1. Moore, B.W. (1965) A soluble protein characteristic of the nervous system.
    Biochem Biophys Res Commun. 19 (6): 739-44.