IL-1F5 antibody

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Rabbit anti Bovine Interleukin-1F5:Biotin

Rabbit anti Bovine Interleukin-1F5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2377B E 50 µg
AHP2377 E WB 0.1 mg
Rabbit anti Bovine interleukin-1F5 antibody recognizes bovine interleukin-1F5 (IL-1F5), also known as interleukin-36 receptor antagonist (IL-36RA), a 17.1 kDa protein belonging to the IL-36 subfamily/IL-1 family of cytokines. IL-1F5/IL-36RA appears to have an inflammatory function. Studies in mice and humans indicate that IL-36 cytokines are generally found in skin tissue and in these species IL-36RA appears to have a role in combating psoriasis (Tsutsui H.et al. 2015).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine interleukin-1F5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant bovine interleukin-1F5
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
Q0VC52 Related reagents
Regulatory
For research purposes only

Applications of IL-1F5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.05ug/ml 0.5ug/ml
ELISA 1ug/ml 5ug/ml
Western Blotting 0.1ug/ml 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection antibody in a sandwich ELISA together with AHP2377 as the capture reagent. Recombinant Bovine interleukin-1F5 (PBP029) may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2377B as the detection reagent. Recombinant Bovine interleukin-1F5 (PBP029) may be used as a standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

Further Reading

  1. Tsutsui, H. et al. (2015) Interleukin-1 Family Cytokines in Liver Diseases.
    Mediators Inflamm. Vol 2015, Article ID 630265