IgA antibody

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Sheep anti Bovine IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI20 E ID 1 mg
Sheep anti Bovine IgA antibody recognizes bovine IgA and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis.

Product Details

Target Species
Bovine
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgA were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for IgA antibody

  1. Morton, H.C. et al. (2004) Cloning and characterization of an immunoglobulin A Fc receptor from cattle.
    Immunology. 111 (2): 204-11.
  2. Subharat, S. et al. (2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.
    Vet Immunol Immunopathol. 164 (3-4): 201-7.
  3. Facciuolo, A. et al. (2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
    PLoS One. 11 (7): e0158747.
  4. Lee, A. et al. (2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.
    J Comp Pathol. 152 (2-3): 201-5.
  5. González-Hernández, A. et al. (2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
    Sci Rep. 6: 29496.