Glutathione Peroxidase antibody

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Sheep anti Bovine Glutathione Peroxidase:Alk. Phos.

Sheep anti Bovine Glutathione Peroxidase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4690-3999A E 1 ml
4690-3999G E 1 ml
Sheep anti Bovine glutathione peroxidase antibody detects human glutathione peroxidise (GSH-Px), a selenium-containing tetrameric enzyme found in mammalian erythrocytes. GSH-Px has an important role in protecting cellular and subcellular membranes from oxidative damage, and functions to detoxify peroxide though the reduction of hydrogen peroxide and organic peroxides.

An increase in GSH-Px in tumour cells may be associated with resistance to drugs that act by generating reactive oxygen species, for example doxorubicin.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine glutathione peroxidase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Glutathione peroxidase from bovine erythrocytes.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00435 Related reagents
Entrez Gene
GPX1 Related reagents
GO Terms
GO:0004602 glutathione peroxidase activity
GO:0005737 cytoplasm
GO:0055114 oxidation-reduction process
GO:0006979 response to oxidative stress
Regulatory
For research purposes only

Applications of Glutathione Peroxidase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/250
ELISA 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Glutathione Peroxidase antibody

  1. Kankofer, M. et al. (2013) The presence of SOD 1 and GSH-Px in bovine retained and properly released foetal membranes.
    Reprod Domest Anim. 48 (4): 699-704.

Further Reading

  1. MILLS, G.C. (1957) Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown.
    J Biol Chem. 229 (1): 189-97.