Carboxypeptidase A1 antibody

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Rabbit anti Bovine Carboxypeptidase A1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2054 E IF P WB 50 µg
Rabbit anti Bovine Carboxypeptidase A1 antibody recognizes bovine Carboxypeptidase A1, a 309 amino acid monomeric pancreatic extopeptidase derived by cleavage of the propeptide. Carboxypeptidase A1 belongs to the peptidase M14 family (UniProt: P00370).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Antiserum Preparation
Antiserum to bovine carboxypeptidase A1 was raised by repeated immunisation of rabbits with highly purified antigen. IgG fraction was prepared from whole serum by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Carboxypeptidase A1 from bovine pancreas.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00730 Related reagents
Entrez Gene
CPA1 Related reagents
GO Terms
GO:0004181 metallocarboxypeptidase activity
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
Regulatory
For research purposes only

Applications of Carboxypeptidase A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 5ug/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Bovine pancreas

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for Carboxypeptidase A1 antibody

  1. Fazio, E.N. et al. (2011) Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice.
    BMC Cell Biol. 12: 17.