Carboxypeptidase A1 antibody
Rabbit anti Bovine Carboxypeptidase A1 antibody used for the evaluation of carboxypepridase and pro-carboxypeptidase in mive tissue lysates by western blotting.
Image caption:
STC2 over-expression reduces severity of acinar cell damage during cerulein-induced pancreatic injury. (A) Analysis of serum amylase levels four hours into CIP treatment show that serum amylase levels rise approximately 3 fold in WT mice when compared to saline (•) whereas the increase in STC2Tg mice is significantly lower at 2-fold (■; * p < 0.01). (B) Tissue edema analysis revealed significant increases for both WT and STC2Tg tissue 4 hours into CIP (black bars) when compared to saline controls (white bars; n = 4 animals). No difference between genotypes was observed. (C) Representative Western blot for procarboxypeptidase (47 kDa) and carboxypeptidase (CPA; 33 kDa) or trypsinogen four hours after initial saline (Sal.) or cerulein (CIP) injections showed decreased accumulation of activate CPA in STC2Tg mice four hours into CIP. Trypsinogen levels do not differ between genotypes. (D) Quantification by densitometry (graph) comparing the ratio of cleaved (active; 33 kDa) to total CPA revealed a significant increase in WT tissue and, to a significantly lower extent, in STC2Tg tissue (n = 4 animals, letters represent statistically different values; p < 0.05). IF analysis for β-catenin (E-H) or Cx32 (I-L) in wild type (WT) or STC2Tg (Tg) mice four hours after initial injection of saline (E, G, I, K) or cerulein (F, H, J, L) showing decreased accumulation only in WT tissue during CIP. (M) Representative Western blot analysis on pancreatic protein extracts 4 hours after initial saline or cerulein (CIP) injection confirm decreased β-catenin and Cx32 accumulation only in WT-CIP treated tissue (n = 4).
From: Fazio EN, Dimattia GE, Chadi SA, Kernohan KD, Pin CL. Stanniocalcin 2 altersPERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice.
BMC Cell Biol. 2011 May 5;12:17.
Rabbit anti Bovine Carboxypeptidase A1 antibody recognizes bovine Carboxypeptidase A1, a 309 amino acid monomeric pancreatic extopeptidase derived by cleavage of the propeptide. Carboxypeptidase A1 belongs to the peptidase M14 family (UniProt: P00370).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig fraction - liquid
- Antiserum Preparation
- Antiserum to bovine carboxypeptidase A1 was raised by repeated immunisation of rabbits with highly purified antigen. IgG fraction was prepared from whole serum by ammonium sulphate precipitation.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Immunogen
- Carboxypeptidase A1 from bovine pancreas.
- Approx. Protein Concentrations
- Total protein concentration 1.0 mg/ml.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P00730 Related reagents
- Entrez Gene
- CPA1 Related reagents
- GO Terms
- GO:0004181 metallocarboxypeptidase activity
- GO:0005615 extracellular space
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- Regulatory
- For research purposes only
Applications of Carboxypeptidase A1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Paraffin | 5ug/ml | ||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Bovine pancreas
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* | ||
| Histar Detection System | STAR3000A | 50 Tests | C P | ||
| Histar Detection System | STAR3000B | 150 Tests | C P | ||
| Histar Detection System | STAR3000C | 500 Tests | C P |
Product Specific References
References for Carboxypeptidase A1 antibody
-
Fazio, E.N. et al. (2011) Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice.
BMC Cell Biol. 12: 17.
