Carboxypeptidase A1 antibody

Rabbit anti Bovine Carboxypeptidase A1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Carboxypeptidase A1

Product Code Applications Pack Size List Price Your Price Qty
AHP2054
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Rabbit anti Bovine Carboxypeptidase A1 antibody recognizes bovine Carboxypeptidase A1, a 309 amino acid monomeric pancreatic extopeptidase derived by cleavage of the propeptide. Carboxypeptidase A1 belongs to the peptidase M14 family (UniP rel="noopener"rot: P00370).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Antiserum Preparation
Antiserum to bovine carboxypeptidase A1 was raised by repeated immunisation of rabbits with highly purified antigen. IgG fraction was prepared from whole serum by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Carboxypeptidase A1 from bovine pancreas.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 5ug/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Bovine pancreas

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

References for Carboxypeptidase A1 antibody

  1. Fazio, E.N. et al. (2011) Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice.
    BMC Cell Biol. 12: 17.

RRID
AB_10556638
UniProt
P00730
Entrez Gene
CPA1
GO Terms
GO:0004181 metallocarboxypeptidase activity
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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