IFN Beta antibody

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Rabbit anti Chicken Interferon Beta:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP944Z
Datasheet
E FN IP WB
MSDS
0.5 mg loader

Rabbit anti Chicken Interferon beta antibody recognizes chicken Interferon beta (chIFN-beta). Reports suggest that chIFN exhibits properties similar to mammalian IFN type I (IFNI).

Functional studies revealed the presence of two distantly related IFNI genes, designated as IFN alpha and IFN beta, found on chicken chromosome Z. The serological distinction between these two IFNs has been verified using neutralisation assays, and there is evidence that the antiviral activity of chIFN-beta is approximately ten-fold less compared to chIFN-alpha. This would suggest that chIFNbeta might have a different physiological role, though chIFN-beta is expressed strongly in virus-infected cells. Rabbit anti Chicken Interferon beta antibody (AHP944Z) does not cross react with IFN gamma.

This antiserum neutralizes chIFN-beta and can be used to discriminate IFN alpha and IFN beta bioactivities, (Schwarz, H.et al.).

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interferon Beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Immunogen
Recombinant chicken Interferon Beta.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q90873 Related reagents
Entrez Gene
IFNB Related reagents
GO Terms
GO:0006952 defense response
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of IFN Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP944Z detects a band of approximately 20 kDa.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for IFN Beta antibody

  1. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
    J Interferon Cytokine Res. 24 (3): 179-84.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
    J Biol Chem. 271 (13): 7635-9.