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Calreticulin antibody

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Goat anti Calreticulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP516
Datasheet
C WB
SDS
0.1 ml loader

Goat anti calreticulin antibodyrecognises calreticulin, a 418 aminoacid ~60 kDa Ca2+ binding chaperone involved in protein modification and folding within the endoplasmic reticulum (UniProt: P15253).

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to calreticulin were raised by repeated immunisation of goats with highly purified antigen.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified rabbit skeletal muscle calreticulin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P15253
Entrez Gene
CALR
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0006457 protein folding
GO:0005529 sugar binding
GO:0005788 endoplasmic reticulum lumen
Regulatory
For research purposes only

Applications of Calreticulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50 1/70
Immunoprecipitation
Western Blotting 1/300 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for Calreticulin antibody

  1. Milner, R.E. et al. (1991) Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum.
    J Biol Chem. 266 (11): 7155-65.
  2. Michalak, M. et al. (1991) Identification and immunolocalization of calreticulin in pancreatic cells: no evidence for "calciosomes".
    Exp Cell Res. 197 (1): 91-9.
  3. Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
    J Immunol. 185 (8): 4738-49.

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