Calreticulin antibody
No associated image currently available
Goat anti calreticulin antibodyrecognises calreticulin, a 418 aminoacid ~60 kDa Ca2+ binding chaperone involved in protein modification and folding within the endoplasmic reticulum (UniProt: P15253).
Product Details
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Human Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to calreticulin were raised by repeated immunisation of goats with highly purified antigen.
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified rabbit skeletal muscle calreticulin.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15253
- Entrez Gene
- CALR
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005509 calcium ion binding
- GO:0006457 protein folding
- GO:0005529 sugar binding
- GO:0005788 endoplasmic reticulum lumen
- Regulatory
- For research purposes only
Applications of Calreticulin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/50 | 1/70 | |
| Immunoprecipitation | |||
| Western Blotting | 1/300 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for Calreticulin antibody
-
Milner, R.E. et al. (1991) Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum.
J Biol Chem. 266 (11): 7155-65. -
Michalak, M. et al. (1991) Identification and immunolocalization of calreticulin in pancreatic cells: no evidence for "calciosomes".
Exp Cell Res. 197 (1): 91-9. -
Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185 (8): 4738-49.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
