Goat anti calreticulin antibodyrecognises calreticulin, a 418 aminoacid ~60 kDa Ca2+ binding chaperone involved in protein modification and folding within the endoplasmic reticulum (UniProt: P15253).
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rabbit Human Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to calreticulin were raised by repeated immunisation of goats with highly purified antigen.
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Purified rabbit skeletal muscle calreticulin.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P15253 Related reagents
- Entrez Gene
- CALR Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005509 calcium ion binding
- GO:0006457 protein folding
- GO:0005529 sugar binding
- GO:0005788 endoplasmic reticulum lumen
- For research purposes only
Applications of Calreticulin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/70|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Rabbit anti Goat IgG (Fc):FITC||STAR122F||1 mg||F|
|Rabbit anti Goat IgG (Fc):HRP||STAR122P||1 mg||C E WB|
Product Specific References
References for Calreticulin antibody
Milner, R.E. et al. (1991) Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum.
J Biol Chem. 266 (11): 7155-65.
Michalak, M. et al. (1991) Identification and immunolocalization of calreticulin in pancreatic cells: no evidence for "calciosomes".
Exp Cell Res. 197 (1): 91-9.
Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185 (8): 4738-49.