Blue Fluorescent Protein antibody

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Rabbit anti Blue Fluorescent Protein

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
50 µg loader

Rabbit anti BFP antibody recognizes the blue fluorescent protein (BFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. BFP is a ~29 kDa protein that is optimally excited at a wavelength of 380 nm and emits light at 448 nm (Day and Davidson 2009). In multi-color and FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, BFP is often used in combination with EGFP (enhanced green fluorescent protein) (Day and Davidson 2009).

Product Details

Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to blue fluorescent protein (BFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.03% ProclinTM300
E. coli expressed recombinant blue fluorescent protein
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

For research purposes only

Applications of Blue Fluorescent Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti BFP antibody is predicted to recognize a band of approximately 29 kDa on recombinant BFP. Observed band size is 29-36 kDa.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti mCherry AHP2326 WB 50 µg loader
Rabbit anti Green Fluorescent Protein AHP2984 WB 50 µg loader
Rabbit anti Cyan Fluorescent Protein AHP2986 WB 50 µg loader
Rabbit anti Red Fluorescent Protein AHP2987 WB 50 µg loader
Mouse anti mCherry MCA6020 WB 50 µg loader

Product Specific References

Further Reading

  1. Day, R.N. & Davidson, M.W. (2009) The fluorescent protein palette: tools for cellular imaging.
    Chem Soc Rev. 38 (10): 2887-921.

Fluorescent Spectraviewer

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How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra