8-Hydroxyguanosine antibody

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Goat anti 8-Hydroxyguanosine

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP592 E P 0.1 ml
Goat anti 8-Hydroxyguanosine antibody recognises 8-hydroxyguanosine (8-OG), a modified base which occurs in DNA as a result of oxidative stress. 8-hydroxy-2'-deoxyguanosine (8-OHdG) has become a sensitive marker of oxidative damage in cellular DNA and has been reported to be excreted in the urine. Levels of 8-OHdG have been shown to increase on exposure to ionizing radiation and tobacco smoke. Goat anti 8-Hydroxyguanosine antibody cross reacts completely with 8-OHdG.

Goat anti 8-Hydroxyguanosine antibody does not cross react with other naturally occurring nucleotides.

Product Details

Target Species
Chemical
Product Form
Serum - liquid
Antiserum Preparation
Antisera to 8-Hydroxyguanosine were raised by repeated immunisation of goat with highly purified antigen.
Preservative Stabilisers
0.08%Sodium Azide
Immunogen
8-Hydroxyguanosine - conjugate

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of 8-Hydroxyguanosine antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100,000 1/250,000
Immunohistology - Paraffin 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for 8-Hydroxyguanosine antibody

  1. Dwivedi, S. et al. (2012) Butachlor induced dissipation of mitochondrial membrane potential, oxidative DNA damage and necrosis in human peripheral blood mononuclear cells
    Toxicology. 302: 77-87.
  2. Dwivedi, S. et al. (2012) Characterization of coal fly ash nanoparticles and induced oxidative DNA damage in human peripheral blood mononuclear cells.
    Sci Total Environ. 437: 331-8.