4-Hydroxynonenal antibody

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Goat anti 4-Hydroxynonenal

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1251 E WB 0.1 ml
Goat anti 4-hydroxynonenal antibody recognises 4-hydroxynonenal (HNE). HNE is a ~65 kDa, highly reactive aldehyde released upon oxidation of omega-6-insaturated fatty acids, often by free radicals. HNE acts to spread and increase the initial effects of free radical events. It binds nucleic acids, phospholipids and sulfhydryl, histidine and lysine groups. These conjugates cause the cytotoxic effects (including cell death) that occur during oxidative stress due to hydrogen peroxide, superoxide, UV, heat and oxidant chemicals. HNE plays a role in the pathogenesis of diseases, stimulating fibrogenesis and inflammation. It is thought to act as a sensor of external stimuli, inducing the stress response by modulating membrane receptors such as Epidermal growth factor receptor or Fas.

It is thought that constitutive levels of HNE may be needed for normal cell functions as decreased HNE levels are associated with cell proliferation and increased HNE levels with elevated apoptosis.

Product Details

Target Species
Broad
Product Form
Serum - liquid
Antiserum Preparation
Antisera to 4-hydroxynonenal were raised by repeated immunisations of goats with highly purified antigen.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
4-hydroxynonenal conjugate.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of 4-Hydroxynonenal antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10000
Western Blotting 1/3000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

Further Reading

  1. Boon, P. et al. (1999) Glutathione Conjugation of 4-Hydroxy-trans-2,3-nonenal in the Rat in Vivo, the Isolated Perfused Liver and Erythrocytes.
    Toxicol. Appl. Pharmacol. 159:214-23.
  2. Dwivedi S, et al. (2007) Role of 4-hydroxynonenal and its metabolites in signaling.
    Redox. Rep. 12:4-10.