Neurofilament L Antibody | DA2

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Neurofilament L Antibody | DA2 gallery image 1

Immunostaining of cultured rat neurons with anti-mouse neurofilament L (green) and anti-rabbit alpha interferon

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Neurofilament L Antibody | DA2 gallery image 2

Western blot of rat cortex lysate showing specific immunolabeling of the ~68 kDa NF-L protein

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  • Neurofilament L Antibody | DA2 thumbnail image 1
  • Neurofilament L Antibody | DA2 thumbnail image 2
  • Mouse anti Pig Neurofilament L
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  • Product Type
    Monoclonal Antibody
  • Clone
    DA2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4751IF, WBdatasheet pdfdatasheet pdf0.1 ml
    MCA4751
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Pig Neurofilament L antibody, clone DA2 recognizes Neurofilament light polypeptide, also known as neurofilament L, NF-L, 68 kDa neurofilament protein or Neurofilament triplet L protein. NF-L is a 548 amino acid ~68 kDa neurofilament. NF-L is the most abundant of the three nurofilament proteins (NF-H, NF-M and NF-L). Neurofilaments are 10nm or intermediate filaments found in neurons, with especially high concentrations along the axons, where they appear to control axonal diameter.

      In humans defects in the NEFL gene are the cause of Charcot-Marie-Tooth disease 1F (CMT1F), a demyelinating condition of the peripheral nervous system characterized by progressive weakness and atrophy (Jordanova et al. 2003) and the related condition Charcot-Marie-Tooth disease 2E (CMT2E), characterized by loss of muscle tissue and touch sensation with axonal degeneration in the absence of myelin alteration (De Jonghe et al. 2001).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/100
      Western Blotting1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4751 detects a band of approximately 68kDa in rat cortex lysate.
    • Instructions For Use

    Additional Neurofilament L Antibody Formats

    Formats Clone Applications Sizes available
    Neurofilament L Antibody : S/N DA2 IF, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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