Insulin Antibody

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Insulin Antibody gallery image 1

Immunoperoxidase staining of human pancreas using Guinea Pig anti Pig Insulin antibody (5330-0104G) followed by Goat anti Guinea Pig IgG (H/L):HRP (AHP863P). Low power

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Insulin Antibody gallery image 2

Immunoperoxidase staining of human pancreas using Guinea Pig anti Pig Insulin antibody (5330-0104G) followed by Goat anti Guinea Pig IgG (H/L):HRP (AHP863P). Med power

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Insulin Antibody gallery image 3

Immunoperoxidase staining of human pancreas using Guinea Pig anti Pig Insulin antibody (5330-0104G) followed by Goat anti Guinea Pig IgG (H/L):HRP (AHP863P). High power

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Insulin Antibody gallery image 4

Immunoperoxidase staining of human pancreas using Guinea Pig anti Pig Insulin antibody (5330-0104G) followed by Goat anti Guinea Pig IgG (H/L):HRP (AHP863P). Med power

Enlarge
Insulin Antibody gallery image 5

Immunoperoxidase staining of human pancreas using Guinea Pig anti Pig Insulin antibody (5330-0104G) followed by Goat anti Guinea Pig IgG (H/L):HRP (AHP863P). High power

Enlarge
Insulin Antibody gallery image 6

Immunofluorescence staining of human pancreas using Guinea Pig anti Pig Insulin (5330-0104G) in green showing co-localization with amylin (MCA1126) in red. Merged image on right

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5330-0104GC, E, Pdatasheet pdfdatasheet pdf50 µl
    5330-0104G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Guinea pig anti Pig insulin antibody recognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the ‘C chain’) and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of 6kDa in western blotting of porcine, bovine and lapine samples (Schechter et al. 1992).

      Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schiel et al. 2006).

      Guinea pig anti pig insulin antibody has been successfully used to demonstrate the presence og insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Ortsäter et al. 2012).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to pig insulin were raised by repeated immunisations of Guinea Pigs with highly purified antigen. Ig fraction prepared from whole serum by ammonium sulphate precipitation.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Native.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of peptidyl-tyrosine phosphorylation
      glucose metabolic process
      positive regulation of insulin receptor signaling pathway
      hormone activity
      positive regulation of DNA replication
      extracellular region
      positive regulation of protein autophosphorylation
      positive regulation of cell migration
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen1/501/1600
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Pancreas.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Insulin Antibody Formats

    Formats Applications Sizes available
    Insulin Antibody : Purified C, E, P 50 µl
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Pancreas.
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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