IL-17A
Recombinant Pig Interleukin-17A
- Product Type
- Recombinant Protein
- Specificity
- IL-17A
Antibody Quality and Performance Guarantee
| Recombinant pig interleukin-17A (IL-17A) is a recombinant protein corresponding to aa 24-153 of native porcine IL-17A. Native porcine IL-17A is a 17.3 kDa regulatory cytokine belonging to the IL-17 cytokine family. Porcine IL-17A is produced primarily in activated T cells and is found at high levels in porcine intestinal tissues including the jejunum and ileum. Porcine IL-17A functions in the mediation of proinflammatory and induces the production of other cytokines. |
- Target Species
- Pig
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 14.9 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IL-17A
-
Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
Res Vet Sci. 131: 266-300.
- UniProt
- Q60I29
- Entrez Gene
- IL17A
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
View more products with IL-17 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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