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IL-13

Recombinant Pig Interleukin-13

Product Type
Recombinant Protein
Specificity
IL-13

Product Code Applications Pack Size List Price Your Price Qty
PPP026
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 5 µg loader
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Recombinant Pig interleukin-13 (IL-13) is a recombinant protein corresponding to aa 11-131 of native pig IL-13.

Pig IL-13 is an immunoregulatory cytokine produced by activated T cells. IL-13 has a range of activities that include the stimulation of B cell proliferation and immunoglobulin production, as well as the induction of differentiation in both B cells and cells of the monocytic lineage.

Target Species
Pig
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
13.2 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2394 as a coating antibody and AHP2394B as a detection antibody.

References for IL-13

  1. Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
    Res Vet Sci. 131: 266-300.

UniProt
Q95J68
Entrez Gene
IL13
GO Terms
GO:0005125 cytokine activity
GO:0005144 interleukin-13 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response

PPP026

135653 150188 160704 163230

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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