IFN Gamma Antibody | 6477

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Detection of porcine interferon gamma with Human anti Porcine Interferon Gamma (HCA090)

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  • Human anti Pig Interferon Gamma
  • Human anti Pig Interferon Gamma
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    6477
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA090Edatasheet pdfdatasheet pdf0.1 mg
    HCA090
    HCA090AEdatasheet pdfdatasheet pdf50 µg
    HCA090A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Human anti Pig Interferon gamma antibody, clone 6477 recognizes porcine interferon gamma (IFN gamma). IFN gamma is a type II interferon, which is structurally unrelated to type I interferons. Interferons regulate innate and adaptive immunity to viral infection. IFN gamma has anti viral activity and is secreted by a wide range of cells including T-cells, B-cells, NK cells and professional antigen presenting cells such as macrophages and dendritic cells.

      HCA090 is recommended for use as a capture antibody in ELISA assays to detect native porcine IFN gamma. MCA1783B is recommended as a detection reagent.
    • Intended Use
    • Target Species
      Pig
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Recombinant porcine interferon gamma
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      response to virus
      cytokine activity
      immune response
      interferon-gamma receptor binding
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/201/400
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/201/400

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Purified 6477 E 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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