Glucagon Antibody

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Glucagon Antibody gallery image 1

Immunohistochemical detection of glucagon in human pancreas using Sheep anti Pig glucagon antibody (4660-0930) followed by detection using the HISTAR detection kit. Low power

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Glucagon Antibody gallery image 2

Immunohistochemical detection of glucagon in human pancreas using Sheep anti Pig glucagon antibody (4660-0930) followed by detection using the HISTAR detection kit. High power

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Glucagon Antibody gallery image 3

Immunohistochemical detection of glucagon in human pancreas using Sheep anti Pig glucagon antibody (4660-0930) followed by detection using the HISTAR detection kit. High power

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  • Sheep anti Pig Glucagon (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4660-0930C, E, IF, P, Rdatasheet pdfdatasheet pdf0.5 ml
    4660-0930
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Pig glucagon antibody recognizes porcine glucagon, a 29 amino acid peptide produced bu cleavage of preproglucagon. Glucagon plays acentral role in glucose metabolism and homeostasis through increasing glucogenesis and reduction of glycolysis. Glucagon is synthesized by the A cells of the endocrine pancreas (UniProt: P01274).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified Ig - liquid
    • Reconstitution
    • Preparation
      Purified Ig prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Zinc glucagon-BSA.
    • Purity
    • Approx. Protein Concentrations
      5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      extracellular region
      hormone activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/50001/50,000
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Radioimmunoassays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Glucagon Antibody Formats

    Formats Applications Sizes available
    Glucagon Antibody : Purified C, E, IF, P, R 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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