CSF1R Antibody | ROS8G11

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CSF1R Antibody | ROS8G11 gallery image 1

Staining of pig alveolar macrophages with Mouse anti Pig CSF1R (MCA5957GA) followed by FITC conjugated Goat anti Mouse IgG (STAR117F) following permeabilization with Leucoperm (BUF09)

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CSF1R Antibody | ROS8G11 gallery image 2

Staining of pig alveolar macrophages with Mouse anti Pig CSF1R (MCA5957F)

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CSF1R Antibody | ROS8G11 gallery image 3

Staining of pig alveolar macrophages with Mouse anti Pig CSF1R (MCA5957APC)

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CSF1R Antibody | ROS8G11 gallery image 4

Staining of dog bone marrow derived macrophages (BMDMs) using Mouse anti pig CSF1-R, clone ROS 8G11 (MCA5957). Image courtesy of the Roslin Institute

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  • CSF1R Antibody | ROS8G11 thumbnail image 1
  • CSF1R Antibody | ROS8G11 thumbnail image 2
  • CSF1R Antibody | ROS8G11 thumbnail image 3
  • CSF1R Antibody | ROS8G11 thumbnail image 4
  • Mouse anti Pig CSF1R
  • Mouse anti Pig CSF1R:APC
  • Mouse anti Pig CSF1R:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ROS8G11
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5957GAE, F *, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5957GA
    MCA5957FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5957F
    MCA5957APCFdatasheet pdfdatasheet pdf100 Tests
    MCA5957APC
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CSF1R, clone ROS8G11, recognizes the porcine homologue of human CSF1R, also known as CSF-1R, M-CSF receptor, CD115 and c-fms. CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010).

      CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceau et al. 2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffat et al. 2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, it has been shown to recognise canine CD115.
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanno
      Horseno
      Sheepno
      Bovineno
      Dogyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Recombinant pCSF1R-Ig / Fc fusion protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the Ba/F3 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of reconstitution
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Western Blotting
      Flow Cytometry(1)1/251/200
      (1)
      Results may be enhanced using permeabilisation. Bio-Rad recommends the use of LeucopermTM (product code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CSF1R Antibody Formats

    Formats Clone Applications Sizes available
    CSF1R Antibody : APC ROS8G11 F 100 Tests
    CSF1R Antibody : FITC ROS8G11 F 0.1 mg
    CSF1R Antibody : Purified ROS8G11 E, F *, IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)STAR117D405GA0.1 mgF, IF
      STAR117D405GA
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:APCMCA929APC100 TestsF
        MCA929APC
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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