CSF1-Fc

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Recombinant Porcine CSF1-Fc

Day 5 porcine monocyte derived macrophages differentiated in 1 µg/ml of Recombinant Porcine CSF1-Fc.

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  • Recombinant Pig CSF1-Fc
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PPP031FNdatasheet pdfdatasheet pdf1 mg
    PPP031
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant Pig CSF1-Fc is a recombinant fusion protein of porcine macrophage colony-stimulating factor (CSF1) and the Fc region of porcine IgG1a.

      Macrophage colony-stimulating factor (CSF1) is the main regulator of macrophage differentiation in all vertebrates (mammals, birds and fish). Mutations in CSF1 and CSF1 receptor (CSF1-R) genes support the view that CSF1-dependent macrophages are essential for normal development and homeostasis. Therefore, numerous potential therapeutic applications of CSF1 have been envisaged (Gow et al. 2010), (Hume et al. 2012). Recombinant CSF1 has been extensively used in studies of macrophage biology, in vitro, to drive the production of macrophages from bone marrow progenitors in culture and to promote the maturation of monocytes (Schroder et al. 2012).

      CSF1 has diverged between species, and there is limited cross reactivity between mouse and human. However, the recombinant pig CSF1-Fc protein is active in mouse experiments. The bioactive CSF1 molecule is only 150 amino acids, and when injected, has a short half-life in circulation. The fusion protein of pig CSF1 with the Fc region of pig IgG1a was produced in order to extend the half-life, permitting analysis of the role of the protein in macrophage homeostasis and function. The Fc component had no independent biological activity. And the administration of CSF1-Fc in mice produced a large increase in blood monocytes, and in macrophage numbers throughout the body. The protein was also shown to be safe and efficacious when injected into pigs (Gow et al. 2014).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified recombinant protein - liquid
    • Reconstitution
    • Preparation
      Recombinant protein expressed in the egg white of transgenic hens (original stock ISA brown and NovoGen brown), and purified by protein A and size exclusion chromatography.
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      The observed molecular weight by SDS-PAGE is 98 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CSF1-Fc Formats

    Formats Applications Sizes available
    CSF1-Fc : Rec. Protein FN 1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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