CD8 Beta Antibody | PPT23

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CD8 Beta Antibody | PPT23 gallery image 1

Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain (MCA5954GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

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CD8 Beta Antibody | PPT23 gallery image 2

Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC (MCA5954F)

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CD8 Beta Antibody | PPT23 gallery image 3

Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE (MCA5954PE)

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CD8 Beta Antibody | PPT23 gallery image 4

Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse anti porcine CD8b (MCA5954PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD8 Beta Antibody | PPT23 gallery image 5

Figure A. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD8 Beta Chain:RPE
  • Mouse anti Pig CD8 Beta Chain:FITC
  • Mouse anti Pig CD8 Beta Chain
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  • Product Type
    Monoclonal Antibody
  • Clone
    PPT23
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5954FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5954F
    MCA5954GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA5954GA
    MCA5954PEFdatasheet pdfdatasheet pdf100 Tests
    MCA5954PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.

      Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Porcine thymus membrane lysate
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution
      18 months from date of despatch
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Beta Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Beta Antibody : FITC PPT23 F 0.1 mg
    CD8 Beta Antibody : RPE PPT23 F 100 Tests
    CD8 Beta Antibody : Purified PPT23 F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD45RA:FITCMCA1751F0.1 mgF
          MCA1751F
          Mouse anti Pig CD3:FITCMCA5951F0.1 mgF
          MCA5951F
          Mouse anti Pig CD4 AlphaMCA1749GA0.1 mgC *, F
          MCA1749GA
          Mouse anti Pig CD3MCA5951GA0.1 mgC, F, IP, WB
          MCA5951GA
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD4 AlphaMCA1749GA0.1 mgC *, F
          MCA1749GA
          Mouse anti Pig CD3MCA5951GA0.1 mgC, F, IP, WB
          MCA5951GA
          Mouse anti Pig CD4 Alpha:RPEMCA1749PE100 TestsF
          MCA1749PE
          Mouse anti Pig CD3:RPEMCA5951PE100 TestsF
          MCA5951PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig wCD8 Alpha:FITCMCA1223F0.1 mgF
          MCA1223F
          Mouse anti Pig CD4 Alpha:FITCMCA1749F0.1 mgF
          MCA1749F
          Mouse anti Pig CD5:FITCMCA2307F0.1 mgF, IP, WB
          MCA2307F
          Mouse anti Pig wCD8 AlphaMCA1223GA0.1 mgC, F
          MCA1223GA
          Mouse anti Pig CD4 AlphaMCA1749GA0.1 mgC *, F
          MCA1749GA
          Mouse anti Pig CD3MCA5951GA0.1 mgC, F, IP, WB
          MCA5951GA
          Mouse anti Pig wCD8 Alpha:RPEMCA1223PE100 TestsF
          MCA1223PE
          Mouse anti Pig CD4 Alpha:RPEMCA1749PE100 TestsF
          MCA1749PE

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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