CD61 Antibody | JM2E5

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CD61 Antibody | JM2E5 gallery image 1

Staining of porcine peripheral blood platelets with Mouse anti Porcine CD61:FITC (MCA2263F)

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  • CD61 Antibody | JM2E5 thumbnail image 1
  • Mouse anti Pig CD61:FITC
  • Mouse anti Pig CD61
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  • Product Type
    Monoclonal Antibody
  • Clone
    JM2E5
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2263FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2263F
    MCA2263GAC, F, IP, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2263GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Pig CD61 antibody, clone JM2E5 recognizes the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa.

      CD61 is expressed by platelets and megakaryocytes, but not by other haematopoietic cells. The epitope recognized by this antibody is not sensitive to EDTA.

      Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85kDa in porcine platelet lysates by western blotting.
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Dogyes
      Humanyes
      Bovineyes
      Horseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine peripheral blood mononuclear cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD61 Antibody Formats

    Formats Clone Applications Sizes available
    CD61 Antibody : FITC JM2E5 F 0.1 mg
    CD61 Antibody : Purified JM2E5 C, F, IP, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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              References

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