CD52 Antibody | 11/305/44

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CD52 Antibody | 11/305/44 gallery image 1

Staining of pig peripheral blood lymphocytes with Mouse anti Pig CD52 (MCA5974GA) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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CD52 Antibody | 11/305/44 gallery image 2

Staining of pig peripheral blood lymphocytes with Mouse anti Pig CD52:FITC (MCA5974F)

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  • CD52 Antibody | 11/305/44 thumbnail image 1
  • CD52 Antibody | 11/305/44 thumbnail image 2
  • Mouse anti Pig CD52
  • Mouse anti Pig CD52:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    11/305/44
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5974GAF, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA5974GA
    MCA5974FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5974F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD52, clone 11/305/44 is a monoclonal antibody recognizing the porcine homologue of human CD52, a 19 kDa antigen expressed by mature lymphocytes, monocytes and dendritic cells.

      Mouse anti Pig CD52, clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1 (Lunney et al.1994). Further studies have demonstrated that SWC1 is the porcine orthologue to human CD52, which is expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets (Piriou-Guyzlack et al. 2008) & (Leitner et al. 2012). Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999) & (McCollough et al. 1999).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Porcine thymocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD52 Antibody Formats

    Formats Clone Applications Sizes available
    CD52 Antibody : FITC 11/305/44 F 0.1 mg
    CD52 Antibody : Purified 11/305/44 F, P 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)STAR117D405GA0.1 mgF, IF
      STAR117D405GA
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD203a:FITCMCA1973F0.1 mgF
          MCA1973F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD203aMCA1973GA0.1 mgC, F, IP
          MCA1973GA

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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