CD5 Antibody | 1H6/8

//www.bio-rad-antibodies.com/pig-porcine-cd5-antibody-1h6-8-mca2307f.html?RenderPageSpecific=1
CD5 Antibody | 1H6/8 gallery image 1

Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD5: FITC (MCA2307F)

Enlarge
CD5 Antibody | 1H6/8 gallery image 2

Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse anti porcine CD4a (MCA1749PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells

Enlarge
CD5 Antibody | 1H6/8 gallery image 3

Figure A. RPE conjugated mouse anti porcine CD4a (MCA1749PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti porcine CD4a (MCA1749PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
CD5 Antibody | 1H6/8 gallery image 4

Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse anti porcine CD8a (MCA1223PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
CD5 Antibody | 1H6/8 gallery image 5

Figure A. RPE conjugated mouse anti porcine CD8a (MCA1223PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti porcine CD8a (MCA1223PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
CD5 Antibody | 1H6/8 gallery image 6

Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse anti porcine CD8b (MCA5954PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
CD5 Antibody | 1H6/8 gallery image 7

Figure A. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Enlarge
  • CD5 Antibody | 1H6/8 thumbnail image 1
  • CD5 Antibody | 1H6/8 thumbnail image 2
  • CD5 Antibody | 1H6/8 thumbnail image 3
  • CD5 Antibody | 1H6/8 thumbnail image 4
  • CD5 Antibody | 1H6/8 thumbnail image 5
  • CD5 Antibody | 1H6/8 thumbnail image 6
  • CD5 Antibody | 1H6/8 thumbnail image 7
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1H6/8
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2307FF, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2307F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD5, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmuller et al. 1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody (Pescowitz et al. 1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine γ/δ T cells (Saalmuller et al. 1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+).

      No reactivity of Mouse anti pig CD5 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      ConA/PMA activated porcine peripheral blood cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD5 Antibody Formats

    Formats Clone Applications Sizes available
    CD5 Antibody : FITC 1H6/8 F, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

              Write your review

              You may also be interested in...