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CD46 Antibody | JM6C11

CD46 Antibody | JM6C11 gallery image 1

Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD46 (MCA2262)

CD46 Antibody | JM6C11 gallery image 2

Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD46 (MCA2262GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

  • CD46 Antibody | JM6C11 thumbnail image 1
  • CD46 Antibody | JM6C11 thumbnail image 2
  • Mouse anti Pig CD46
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2262GAFdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Pig CD46 antibody, clone JM6C11 recognizes porcine CD46, also known as membrane cofactor protein (MCP). CD46 is a ~45-65 kDa single pass type 1 transmembrane glycoprotein expressed by all peripheral blood cells.

      Mouse anti Pig CD46 antibody, clone JM6C11 inhibits functional MCP cofactor activity as measured by inhibition of cleavage of the α chain of C3 (Pérez De La Lastra et al. 1999).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine peripheral blood mononuclear cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      inner acrosomal membrane
      innate immune response
      complement activation, classical pathway
      integral to membrane
      single fertilization
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10 ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD46 Antibody Formats

    Formats Clone Applications Sizes available
    CD46 Antibody : Purified JM6C11 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD46 Antibody | JM6C11 thumbnail image 1
                • CD46 Antibody | JM6C11 thumbnail image 2
              • Application NameReference Images
                Functional Assays
                Western Blotting


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