CD45 Antibody | MAC323

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CD45 Antibody | MAC323 gallery image 1

Figure A. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse IgG2a isotype control (MCA929) detected with RPE conjugated goat anti mouse IgG (STAR133PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45 (MCA1447) detected with RPE conjugated goat anti mouse IgG (STAR133PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD45 Antibody | MAC323 gallery image 2

Published customer image:
Mouse anti Porcine CD45 antibody, clone MAC323 (MCA1447) used to illustrate inflammatory cells in an implant model by immunohistochemistry
Image caption:
CD45 histology at 6M.
OCT demonstrated the development of a highly heterogeneous neointima with inflammation (F; CD45).

From: van Ditzhuijzen NS, Kurata M, van den Heuvel M, Sorop O, van Duin RWB, Krabbendam-Peters I, et al. (2017)
Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
PLoS ONE 12(9): e0183419.

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CD45 Antibody | MAC323 gallery image 3

Published customer image:
Mouse anti Porcine CD45 antibody, clone MAC323 (MCA1447) used to illustrate inflammatory cells in an implant model by immunohistochemistry on cryostat sections.
Image caption:
Collagen poor areas in peri-strut regions and neointima showing leucocyte infiltration (C, F; CD45).

From: van Ditzhuijzen NS, Kurata M, van den Heuvel M, Sorop O, van Duin RWB, Krabbendam-Peters I, et al. (2017)
Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
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  • Mouse anti Pig CD45 Allotypic Variant
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MAC323
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1447C, F, IP, P, RE*datasheet pdfdatasheet pdf1 mg
    MCA1447
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD45 Allotypic Variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as Leucocyte common antigen (LCA). The antibody has been shown to recognise pigs of strains Large White and German Landrace. Another antibody K252.1E4, recognizes a monomorphic determinant on porcine CD45. When the latter is used in conjunction with MAC323 it is noted that MCA323 stains a subset of antimals that stain with K252.1E4 indicating loss of the epitope in MCA323-ve animals rather than the loss of CD45 antigen. Cross-breeding studies in positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelialn autosomaly dominant pattern (Binns et al. 1995).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Porcine peripheral blood lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/10
      Immunohistology - Paraffin1/10
      Immunoprecipitation
      Immunohistology - Resin(1)1/10
      (1)
      This product requires antigen retrieval using heat treatment of resin sections.

      This product does not require antigen retrieval prior to staining paraffin sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45 Antibody Formats

    Formats Clone Applications Sizes available
    CD45 Antibody : Purified MAC323 C, F, IP, P, RE* 1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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