CD31 Antibody | LCI-4

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CD31 Antibody | LCI-4 gallery image 1

Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD31:FITC (MCA1746F)

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CD31 Antibody | LCI-4 gallery image 2

Published customer image:
Mouse anti Pig CD31 antibody, clone LCI-4 used for the evaluation of PECAM-1 expression on M. suis infected porcine endothelial cells.
Image caption:
CAM expression in M. suis infected endothelial cells. The expression profiles of adhesion molecules on endothelial cells were analyzed using a FACSCanto II cell sorting system. A. Dashed lines represent the isotype control; solid lines represent cells incubated with the negative control preparation; grey histograms represent PEDSV.15 cells incubated with M. suis (1?×?104 cells/mL). B. Percent increase in expression ICAM-1 (CD54), PECAM-1 (CD31), E (CD62E)- and P (CD62P)-selectin in ECs infected with M. suis.

From: Sokoli A, Groebel K, Hoelzle K, Amselgruber WM, Mateos JM, Schneider MK, Ziegler U, Felder KM, Hoelzle LE. Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma.
Vet Res. 2013 Feb 11;44:6.

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CD31 Antibody | LCI-4 gallery image 3

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Mouse anti Pig CD31 antibody, clone LCI-4 (MCA1746GA) used for the detection of blood vessels in normal and diabetic human patient skin by immunohistochemistery of formalin fixed, paraffin embedded tissue sections.
Image caption:
Similar numbers of lymphocytes, blood vessels and proliferating keratinocytes are found in DFS and NFS. Immunostaining and quantification for CD45, a lymphocyte marker, (A, D), CD31, an endothelial cell marker, (B, E) and Ki67, a proliferation marker (C,F), reveal no differences in the number of lymphocytes, blood vessels and proliferating keratinocytes found in DFS and NFS. Ki67 immuno-peroxidase staining for proliferating cells (C) shows that proliferative keratinocytes were located in the basal layer of the skin, as expected. No significant differences were found between DFS and NFS in numbers of CD45+ cells (D), number of CD31+ (E) or Ki67 positive keratinocytes (F). Bar graphs indicate mean and SD.

From: Ramirez HA, Liang L, Pastar I, Rosa AM, Stojadinovic O, Zwick TG, et al. (2015) Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin.
PLoS ONE 10(8): e0137133.

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CD31 Antibody | LCI-4 gallery image 4

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Mouse anti Pig CD31 antibody, clone LCI-4 used for the detection of vascular endothelium in pigs by immunofluorescence.
Image caption:
VECs produce, release, and actively cleave von Willebrand factor. (A) Non-stimulated VECs seeded on hydrogels (3.4 kDa) functionalized with RKR (left) or RGDS (right) stained for CD31 (red) and VWF (green). Nucleus was stained with DAPI (blue). Scale bar = 50 μm. (B) Quantification of rapidly released VWF and (C) the fraction of cleaved VWF-140 fragments from histamine-stimulated VECs cultured on different molecular weight hydrogels functionalized with RKR or RGDS, or TCPS. Groups not connected by same symbols are significantly different. p < 0.05.

From: Balaoing LR, Post AD, Lin AY, Tseng H, Moake JL, Grande-Allen KJ (2015) Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.
PLoS ONE 10(6): e0130749.

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CD31 Antibody | LCI-4 gallery image 5

Published customer image:
Mouse anti Pig CD31 antibody, clone LCI-4 used for the detection of CD31 expressing cells in porcine retina by immunofluorescence.
Image caption:
Phosphorylated p38 immunofluorescence in the retinal arteries and neuroretina. A: A representative example of phosphorylated p38 (p-p38; green) staining of the retinal arteries is shown. Double staining with the smooth muscle cell marker smooth muscle actin (SMA; red) and the endothelium cell marker CD31 (red) shows that p-p38 is primarily located in the smooth muscle layer. Similar results were seen for sham-operated and ischemia-reperfusion eyes. B: p-p38 (green) was also occasionally detected in the inner nuclear layer of the neuroretina. Double staining with protein kinase Cα (PKCα; red), a bipolar cell marker, showed that p38 was localized to bipolar cell bodies, whereas the p38 protein appears to be associated with the nucleus. The abbreviations used in the figure are outer nuclear layer (ONL), outer plexiform layer (OPL), and inner plexiform layer (IPL).

From: Gesslein B, Håkansson G, Carpio R, Gustafsson L, Perez MT, Malmsjö M. Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion. Mol Vis. 2010 Mar 10;16:392-407.

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CD31 Antibody | LCI-4 gallery image 6

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Mouse anti Pig CD31 antibody, clone LCI-4 used for the evaluation of CD31 expression on porcine differentiated fat cells by flow cytometry.
Image caption:
Expression of cell surface antigens on porcine DFAT cells. Expressions of CD44, CD29, CD90, CD31, and CD34 were detected by FITC or CY3 conjugated antibody of P4 DFAT cells.

From: Peng X, Song T, Hu X, Zhou Y, Wei H, Peng J, Jiang S. Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro.
Biomed Res Int. 2015;2015:673651.

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CD31 Antibody | LCI-4 gallery image 7

Figure A. APC conjugated mouse anti porcine CD31 (MCA1746APC) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated mouse anti porcine CD31 (MCA1746APC) and FITC conjugated mouse anti porcine CD11R3 (MCA2309F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD31:APC
  • Mouse anti Pig CD31:RPE
  • Mouse anti Pig CD31:RPE
  • Mouse anti Pig CD31:FITC
  • Mouse anti Pig CD31:Biotin
  • Mouse anti Pig CD31
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LCI-4
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1746FF, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA1746F
    MCA1746BFdatasheet pdfdatasheet pdf0.1 mg
    MCA1746B
    MCA1746GAC, F, IF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1746GA
    MCA1746APCFdatasheet pdfdatasheet pdf100 Tests
    MCA1746APC
    MCA1746PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1746PE
    MCA1746PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1746PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmambrane region as demonstrated by human CD31 domain deletion mutant protein binding studies (Nasu et al.1999).

      Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute with 0.25ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute with 0.25ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine CD31/human IgGFc fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of reconstitution.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD31 Antibody Formats

    Formats Clone Applications Sizes available
    CD31 Antibody : RPE LCI-4 F 25 Tests | 100 Tests
    CD31 Antibody : FITC LCI-4 F, IF 0.1 mg
    CD31 Antibody : Biotin LCI-4 F 0.1 mg
    CD31 Antibody : APC LCI-4 F 100 Tests
    CD31 Antibody : Purified LCI-4 C, F, IF, IP 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

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