CD2a Antibody | MAC83

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  • Rat anti Pig CD2a
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1156CT, F, FNdatasheet pdfdatasheet pdf0.25 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Pig CD2a, clone MAC83 recognizes pig CD2a, a single pass type 1 transmembrane protein of ~50 kDa. Porcine CD2 is a lymphocyte expressed cellular adhesion receptor, originally identified as a lymphocyte restricted sheep red blood cell receptor (Binns et al. 1982).

      Porcine CD2, also known as LFA-2 interacts with its ligand CD58 also known as LFA-3 in a manner analogous to that seen in mice (Sen et al. 1989).

      Rat anti Pig CD2a, clone MAC83 was clustered to CD2a at the first International Swine CD Workshop based on its cell distribution, antigen immunoprecipitation and its ability to completely inhibit the biding of another anti porcine CD2a clone, MSA4 (Pescowitz et al. 1994).
    • Intended Use
    • Target Species
    • Product Form
      Ascitic Fluid - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Pig T lymphoblasts.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 2-5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cell from immunised F344 rats were fused with cells of the Y3.Ag1.2.3 rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays1/2,0001/20,000
      Flow Cytometry1/2000
      Functional Assays1/200
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunohistology - Resin

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD2a Antibody Formats

    Formats Clone Applications Sizes available
    CD2a Antibody : Ascites MAC83 CT, F, FN 0.25 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Further Reading

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