CD18a Antibody | PNK-I

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CD18a Antibody | PNK-I gallery image 1

Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD18a (MCA1972)

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CD18a Antibody | PNK-I gallery image 2

Staining of peripheral blood lymphocytes with Mouse anti Pig CD18a (MCA1972GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD18a Antibody | PNK-I gallery image 3

Figure A. FITC conjugated mouse anti porcine CD18a (MCA1972F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD18a (MCA1972F) and RPE conjugated mouse anti porcine CD27 (MCA5973PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD18a Antibody | PNK-I gallery image 4

Figure A. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse anti porcine CD18a (MCA1972F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD18a
  • Mouse anti Pig CD18a:FITC
  • Mouse anti Pig CD18a:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    PNK-I
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1972GAF, FN*, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1972GA
    MCA1972FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1972F
    MCA1972PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1972PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Pig CD18a, clone PNK-I recognizes porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998). Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin β2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity (Dato and Kim 1990).

      CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules.

      CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor (Chen et al. 2011).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Camelyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Porcine large granular lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      integrin complex
      cell-matrix adhesion
      receptor activity
      binding
      integrin-mediated signaling pathway
      multicellular organismal development
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunoprecipitation
      Functional Assays(1)
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD18a Antibody Formats

    Formats Clone Applications Sizes available
    CD18a Antibody : Purified PNK-I F, FN*, IP 0.1 mg
    CD18a Antibody : FITC PNK-I F 0.1 mg
    CD18a Antibody : RPE PNK-I F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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