CD172a antibody | BL1H7
Mouse anti Porcine CD172a antibody, clone BL1H7 used to identify CD172a expressing porcine monocytes for subsequent analysis of monocyte subsets based on CD14 and SLA II expression by flow cytometry.
Image caption:
Differential binding of CCL2-GFP to monocyte subsets. Porcine PBMC were incubated with CCL2-GFP (CHO cell supernatant) followed by Alexa 488-conjugated rabbit anti-GFP IgG, and then double stained for Tük4 and SLA II. The histograms are gated on the appropriate monocyte subpopulation regions as defined in the dot-plots. Grey histograms show the background fluorescence of monocytes incubated with supernatants from CHO cells transfected with the InvCCL2-GFP construct. 25 000 cells were acquired.
From: Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J.
Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 2010 Sep-Oct;41(5):76.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD172a antibody, clone BL1H7 (MCA2312) used to evaluate SWC3 expression on bone marrow derived dendritic cells by flow cytometry.
Image caption:
Single colour flow cytometry analysis of changes in several immunologically relevant cell surface molecules of bone marrow-derived dendritic cells after 48 h of incubation with different PRRSV strains at a multiplicity of infection of 0.01 (as determined by titration in porcine alveolar macrophages). Grey histograms show cells stained with an irrelevant isotype-matched antibody and a secondary FITC or phycoerithrin conjugated antibody. Blue histograms show uninfected cells and red line histograms correspond to PRRSV-infected cells. Averages and variation coefficients are included. Grey values show the uninfected cell parameters and black values correspond to PRRSV-infected cells. A) SLA-I, SLA-II, CD80/86, CD14; B) SwC3, SwC9, CD163, CD16.
From: Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E.
Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 2011 Jan 18;42:9.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD172a antibody, clone BL1H7 (MCA2312) used to assess CD172a expression on porcine monocytes by flow cytometry.
Image caption:
Chemokine receptor mRNA expression in monocyte subsets. (A) Dot plot of Tük4 versus SLA-II expression in monocytes obtained from PBMC (20 000 cells were acquired). Three subsets can be identified: Tük4+SLA-II−, Tük4+SLA-II+, and Tük4−SLA-II+. (B) The Tük4+SLA-II− and Tük4−SLA-II+ monocyte subsets were magnetically isolated as described in Materials and methods section, and the expression of the indicated markers was analysed by flow cytometry (5 000 cells acquired). (C) Total RNA isolated from PBMC, the whole monocyte population (CD172a+) or the Tük4+SLA-II− and Tük4−SLA-II+ monocyte subsets shown in (B) were reverse transcribed, and the cDNA amplified by PCR with specific primers for GAPDH or different chemokine receptors. PCR products were analysed by 1.5% agarose gel electrophoresis. Data are representative from three independent experiments using different donors.
From: Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J.
Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 2010 Sep-Oct;41(5):76.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD172a antibody, clone BL1H7 used to identify CD172a expressing porcine monocytes for subsequent analysis of monocyte subsets based on CD14 and SLA II expression by flow cytometry.
Image caption:
Differential binding of CCL2-GFP to monocyte subsets. Porcine PBMC were incubated with CCL2-GFP (CHO cell supernatant) followed by Alexa 488-conjugated rabbit anti-GFP IgG, and then double stained for Tük4 and SLA II. The histograms are gated on the appropriate monocyte subpopulation regions as defined in the dot-plots. Grey histograms show the background fluorescence of monocytes incubated with supernatants from CHO cells transfected with the InvCCL2-GFP construct. 25 000 cells were acquired.
From: Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J.
Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 2010 Sep-Oct;41(5):76.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD172a antibody, clone BL1H7 (MCA2312GA) used to detect CD172a expressing cells in porcine tongue by immunohistochemistry on cryostat sections.
Image caption:
Mapping of immune cells in the ventral surface of tongues from rats, dogs, minipigs and monkeys.
Mucosal tissues from animal species were processed for immunohistology analysis, as described in Methods. Slides were stained with the following specific antibodies: anti-MHC-II, anti-CD163 and anti-CD172a to detect and quantify positive cells in tissue sections (magnification x200). Representative photomicrographs of mucosal tissue sections are shown.
From: Thirion-Delalande C, Gervais F, Fisch C, Cuiné J, Baron-Bodo V, Moingeon P, et al. (2017)
Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
PLoS ONE 12(9): e0183398.
doi:10.1371/journal.pone.0183398
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD172 antibody, clone BL1H7 (MCA2312GA) used to label CD172 expressing blood cells by flow cytometry;
Image caption:
Neonatal and adult porcine BDC populations.
(A) Flow cytometry was run on adult (left panel) and neonatal (right panel) BDCs following isolation of the cells. The upper panel represents the size (forward scatter or FSC) and the granulosity of unstained cells (side scatter or SSC), while the bottom panel represents the double staining of adult and neonatal BDCs with CD4 and CD172.
From: Auray G, Facci MR, van Kessel J, Buchanan R, Babiuk LA, Gerdts V (2013)
Porcine Neonatal Blood Dendritic Cells, but Not Monocytes, Are More Responsive to TLRs Stimulation than Their Adult Counterparts.
PLoS ONE 8(5): e59629.
doi: 10.1371/journal.pone.0059629
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD172a, clone BL1H7 (MCA2312GA) used to evaluate CD172a expression on immune cells from porcine skin by flow cytometry.
Image caption:
ZH9F7-Cap targets skin cDC1 s and cDC2s/LCs in vivo. (a) Skin migratory CD163−/lowCD172a+/− cells were selected and analyzed for CADM1 expression, obtaining skin cDC1 (CD163−CADM1+CD172a−/low) and cDC2/LC (CD163−/lowCADM1+CD172ahigh) cells.
From: Melgoza-González, E.A.; Reséndiz-Sandoval, M.; Hinojosa-Trujillo, D.; Hernández-Valenzuela, S.; García-Vega, M.; Mata-Haro, V.; Tepale-Segura, A.; Bonifaz, L.C.; Perez-Torres, A.; Hernández, J.
Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells.
Vaccines 2022, 10, 684.
doi: 10.3390/vaccines10050684.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD172a
- Product Type
- Monoclonal Antibody
- Clone
- BL1H7
- Isotype
- IgG1
- Specificity
- CD172a
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD172a, clone BL1H7 recognizes porcine CD172a, a member of the signal regulatory protein (SIRP) family (Alvarez et al. 2000). Mouse anti Pig CD172a, clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (Haverson et al. 2001;Thacker et al. 2001). CD172a is expressed on monocyte derived dendritic cells (MoDCs) (Facci et al. 2010) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(Facci; Jeong et al. 2010). Mouse anti Pig CD172a, clone BL1H7 immunoprecipitates a single band of ~90-110 kDa from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions (Alvarez et al. 2000). Aberrant expression of CD172a has been noted on porcine leukemias (Sipos et al. 2006) with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a bi-phenotypic pattern as opposed to the more characteristic single population of CD172+ cells seen in normal blood PBMC (Chamorro et al. 2005). Mouse anti Pig CD172a, clone BL1H7 has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues (Domenech et al. 2003). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/20 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
||
| Immunoprecipitation | |
||
| Western Blotting 2 | |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2Clone BL1H7 recognizes porcine CD172a under non-reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
- Histology Positive Control Tissue
- Porcine spleen
- Western Blotting
- Mouse anti Pig CD172a antibody, clone BL1H7 detects a band of approximately 90-115 kDa in alveolar macrophage lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD172a antibody
-
Alvarez, B. et al. (2000) A porcine cell surface receptor identified by monoclonal antibodies to SWC3 is a member of the signal regulatory protein family and associates with protein-tyrosine phosphatase SHP-1.
Tissue Antigens. 55 (4): 342-51. -
Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
Vet Immunol Immunopathol. 94 (1-2): 77-81. -
Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
Vet Immunol Immunopathol. 89 (1-2): 91-8. -
Fraile, L. et al. (2012) Immunomodulatory properties of beta-sitosterol in pig immune responses.
Int Immunopharmacol. 13 (3): 316-21. -
Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17 (4): 503-12. -
Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9. -
Moreno, S. et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 41: 76. -
Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
Immunology. 129: 396-405.
View The Latest Product References
-
Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
Vet Immunol Immunopathol. 104: 131-44. -
Argilaguet, J.M. et al. (2012) DNA vaccination partially protects against African swine fever virus lethal challenge in the absence of antibodies.
PLoS One. 7 (9): e40942. -
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94. -
Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
Cell Biol Int. 36 (12): 1233-8. -
Robinson, S.R. et al. (2015) Broadly neutralizing antibodies against the rapidly evolving porcine reproductive and respiratory syndrome virus.
Virus Res. 203: 56-65. -
Li, J. & Murtaugh, M.P. (2015) Functional analysis of porcine reproductive and respiratory syndrome virus N-glycans in infection of permissive cells.
Virology. 477: 82-8. -
Prims. S. et al. (2016) Intestinal immune cell quantification and gram type classification of the adherent microbiota in conventionally and artificially reared, normal and low birth weight piglets.
J Livestock Sci 185: 1-7. -
Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
Am J Physiol Renal Physiol. 310 (4): F259-71. -
Valekova I et al. (2016) Revelation of the IFNα, IL-10, IL-8 and IL-1β as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model.
J Neuroimmunol. 293: 71-81. -
Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
Am J Physiol Renal Physiol. 310 (4): F259-71. -
Thirion-Delalande, C. et al. (2017) Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
PLoS One. 12 (9): e0183398. -
Auray, G. et al. (2013) Porcine neonatal blood dendritic cells, but not monocytes, are more responsive to TLRs stimulation than their adult counterparts.
PLoS One. 8 (5): e59629. -
Hu, W. et al. (2021) shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus.
Sci Rep. 11 (1): 16377. -
Radlowski, E.C. et al. (2021) Combination-Feeding Causes Differences in Aspects of Systemic and Mucosal Immune Cell Phenotypes and Functions Compared to Exclusive Sow-Rearing or Formula-Feeding in Piglets.
Nutrients. 13(4):1097. -
Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
Vaccines. 10 (5): 684. -
Zhou, L. et al. (2022) Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model.
J Transl Med. 20 (1): 538. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- SWC3
- UniProt
- Q5K4Q3
MCA2312GA
148204
161302
162604
167453
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