CD169 Antibody | 3B11/11

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CD169 Antibody | 3B11/11 gallery image 1

Published originator image:
Mouse anti Porcine CD169 antibody, clone 3B11.11 used for the evaluation of CD169 expression on porcine cells by flow cytometry and immunohistochemistry.
Image caption:
(A) MAb 1F1 and 3B11 recognize an antigen expressed in alveolar macrophages and MoDC. Cells were labeled with 1F1 or 3B11 mAb (black histograms) or with irrelevant isotype-matched mAb (grey histograms). Results are representative of five independent experiments. (B) In the spleen, staining with 1F1 (1) and 3B11 (2) mAb appears associated with the walls of the ellipsoidal vessels that surround the islands of white pulp. (C) In lymph nodes, the antigen was detected in the subcapsular sinus and medullary cords (1) and in the periphery of follicles and some follicular cells (2).

From: Revilla C, Poderoso T, Martínez P, Alvarez B, López-Fuertes L, Alonso F, Ezquerra A, Domínguez J.
Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 2009 May-Jun;40(3):14.

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CD169 Antibody | 3B11/11 gallery image 2

Published customer image:
Mouse anti Porcine CD169 antibody, clone 3B11.11 used for the evaluation of CD169 expression on porcine cells by western blotting and immunoprecipitation.
Image caption:
MAb 1F1 and 3B11 bind to two different epitopes on the Sn molecule. Black histograms show binding of labeled 3B11 or 1F1 mAb on alveolar macrophages after incubation with the indicated unlabeled antibodies. Grey histograms correspond to the negative control staining. Results are representative of at least two independent experiments using cells from different animals.

From: Revilla C, Poderoso T, Martínez P, Alvarez B, López-Fuertes L, Alonso F, Ezquerra A, Domínguez J.
Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 2009 May-Jun;40(3):14.

Enlarge
CD169 Antibody | 3B11/11 gallery image 3

Published customer image:
Mouse anti Porcine CD169 antibody, clone 3B11.11 used for the evaluation of epitope binding in comparison to clone 1F1 by flow cytometry on porcine alveolar macrophages.
Image caption:
MAb 1F1 and 3B11 bind to two different epitopes on the Sn molecule. Black histograms show binding of labeled 3B11 or 1F1 mAb on alveolar macrophages after incubation with the indicated unlabeled antibodies. Grey histograms correspond to the negative control staining. Results are representative of at least two independent experiments using cells from different animals.

From: Revilla C, Poderoso T, Martínez P, Alvarez B, López-Fuertes L, Alonso F, Ezquerra A, Domínguez J.
Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 2009 May-Jun;40(3):14.

Enlarge
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  • Product Type
    Monoclonal Antibody
  • Clone
    3B11/11
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2316FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2316F
    MCA2316GAC, F, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2316GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD169, clone 3B11/11 Is specific for porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003) .

      Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.

      Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011) .

      Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine alveolar macrophages.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Porcine spleen
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD169 Antibody Formats

    Formats Clone Applications Sizes available
    CD169 Antibody : FITC 3B11/11 F 0.1 mg
    CD169 Antibody : Purified 3B11/11 C, F, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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              Source Reference

              References

              Further Reading

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