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NFkB p65

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NFkB p65 Control Peptide

Product Type
Control Peptide
Product Code Applications Pack Size List Price Quantity
PHP073
Datasheet
FN
SDS
50 µg loader

NFκB control peptide can be used to block the specific binding of anti-NFkB p65 (AHP288) with the NFkB p65 subunit. The control peptide should be used at 1μg per 1μl of antiserum used.

Product Details

Target Species
Human
Product Form
Purified peptide - liquid
Preparation
Synthesized peptide is purified by HPLC.
Preservative Stabilisers
0.01%Sodium Azide
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the peptide. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of despatch

More Information

UniProt
Q04206
Entrez Gene
RELA
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003682 chromatin binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0008063 Toll signaling pathway
GO:0042802 identical protein binding
GO:0010224 response to UV-B
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019901 protein kinase binding
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031625 ubiquitin protein ligase binding
GO:0032582 negative regulation of gene-specific transcription
GO:0033613 activating transcription factor binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042301 phosphate binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0047485 protein N-terminus binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050727 regulation of inflammatory response
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus
GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of NFkB p65

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti NFkB p65 AHP288 E GS IF IP WB 0.1 ml loader

Product Specific References

References for NFkB p65

  1. Yao, C. et al. (2009) Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE.
    Biosens Bioelectron. 24 (8): 2499-503.
  2. Zhang, B. et al. (2010) Specific binding DNA-based piezoelectric quartz crystal microbalance biosensor array for the study of NF-κB
    Sensors and Actuators B: Chemical. 149 (1): 259-63.
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