Multiple IgG1/IgG2a Negative Control Antibody

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  • Mouse IgG1:FITC/Rat IgG2a:RPE Negative Control
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  • Product Type
    Negative/Isotype Control
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    DC050Fdatasheet pdfdatasheet pdf50 Tests
    DC050
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • DC050 is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 and rat monoclonals of isotype IgG2a to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.

      N.B. Clone W3/25 recognises a rat cell surface antigen and therefore DC050 cannot be used as a negative control in this species.

      Suitable as negative control for human and dog.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      FITC reagent: IgG1 (MOUSE)
      RPE reagent: IgG2a (RAT)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul to label 106 cells or 100ul whole blood.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Multiple IgG1/IgG2a Negative Control Antibody Formats

    Formats Applications Sizes available
    Multiple IgG1/IgG2a Negative Control Antibody : FITC/RPE F 50 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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