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Versican gag Antibody

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  • Rabbit anti Mouse Versican gag Alpha Domain
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1699IP, P *, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Mouse Versican gag Alpha Domain antibody recognizes murine versican, a large multi-domain chondroitin sulphate proteoglycan secreted by fibroblasts and other types of cells. It contains an N-terminal hyaluronic acid binding domain that has homology with the G1 and G2 link domains of aggrecan which stabilize the binding of aggrecan to hyaluronic acid and to the hyaluronate receptor CD44. In addition, versican also has a lectin-like domain, a C-terminal complement repeat and two EGF-like repeats all of which can be found in aggrecan.

      Rabbit anti Mouse Versican gag Alpha Domain antibody binds to an epitope within the glucosaminoglycan attachment domain-alpha region of murine versican.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      None present
    • Immunogen
      GST fusion protein containing amino acids 535-598 of mouse versican.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate Buffered Saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      heart development
      cell adhesion
      proteinaceous extracellular matrix
      calcium ion binding
      ureteric bud development
      sugar binding
      hyaluronic acid binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting5 ug/ml
      Immunohistology - Paraffin(1)10 ug/ml
      Tissues should be treated with chondroitinase before staining (0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature) prior to blocking and subsequent staining.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Surrounding cartilage or neural tube/brain
    • Immunofluorescence
    • Western Blotting
      Positive control: Newborn mouse brain
    • Instructions For Use

    Additional Versican gag Antibody Formats

    Formats Applications Sizes available
    Versican gag Antibody : Purified IP, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Surrounding cartilage or neural tube/brain
              • Application NameReference Images
                Western Blotting


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