TLR4/MD-2 Antibody | MTS510

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  • Rat anti Mouse TLR4/MD-2
  • Rat anti Mouse TLR4/MD-2:Alexa Fluor® 488
  • Rat anti Mouse TLR4/MD-2
  • Rat anti Mouse TLR4/MD-2:Alexa Fluor® 488
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MTS510
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2154F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2154
    MCA2154A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2154A488
    MCA2154TF, IPdatasheet pdfdatasheet pdf25 µg
    MCA2154T
    MCA2154A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2154A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse TLR4/MD-2 antibody, clone MTS510 recognizes mouse toll-like receptor 4 (TLR4), when associated with MD-2 and co-precipitates MD-2 with TLR4. Rat anti Mouse TLR4/MD-2 antibody, clone MTS510 does not detect TLR4 alone. TLR4 mediates the innate immune response to bacterial lipopolysaccharide through co-operation with MD-2 and CD14.

      Clone Rat anti Mouse TLR4/MD-2 antibody, clone MTS510 has been reported to block LPS-induced cytokine production.

      N.B. Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation (Akashi et al. 2003).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Ba/F3 cell line transfected with mouse TLR4 and MD-2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      positive regulation of JNK cascade
      positive regulation of interferon-gamma production
      positive regulation of stress-activated MAPK cascade
      positive regulation of B cell proliferation
      activation of NF-kappaB-inducing kinase activity
      negative regulation of interleukin-6 production
      positive regulation of tumor necrosis factor production
      cellular response to lipoteichoic acid
      positive regulation of interferon-alpha production
      negative regulation of interferon-gamma production
      transmembrane receptor activity
      lipopolysaccharide receptor activity
      mast cell activation
      positive regulation of platelet activation
      positive regulation of nitric oxide biosynthetic process
      innate immune response
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      nitric oxide production involved in inflammatory response
      lipopolysaccharide receptor complex
      intestinal epithelial structure maintenance
      positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway
      interferon-gamma production
      negative regulation of ERK1 and ERK2 cascade
      positive regulation of interleukin-6 biosynthetic process
      positive regulation of interleukin-12 production
      positive regulation of MHC class II biosynthetic process
      positive regulation of macrophage cytokine production
      positive regulation of nitric-oxide synthase biosynthetic process
      negative regulation of tumor necrosis factor production
      positive regulation of interferon-beta production
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      positive regulation of interleukin-13 biosynthetic process
      positive regulation of interferon-beta biosynthetic process
      negative regulation of interleukin-17 production
      negative regulation of interleukin-23 production
      positive regulation of interleukin-1 production
      activation of MAPK activity
      detection of lipopolysaccharide
      positive regulation of ERK1 and ERK2 cascade
      positive regulation of interleukin-6 production
      positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
      defense response to Gram-negative bacterium
      positive regulation of interleukin-1 biosynthetic process
      positive regulation of interleukin-10 production
      positive regulation of chemokine production
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional TLR4/MD-2 Antibody Formats

    Formats Clone Applications Sizes available
    TLR4/MD-2 Antibody : Alexa Fluor® 488 MTS510 F 25 Tests/0.25ml | 100 Tests/1ml
    TLR4/MD-2 Antibody : Purified MTS510 F, IP 0.25 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488100 Tests/1mlF
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunoprecipitation

              References

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