Sign-R1 Antibody | ER-TR9

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Sign-R1 Antibody | ER-TR9 gallery image 1

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti MouseSIGN-R1 antibody, clone ER-TR9 (MCA2394) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Low power

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Sign-R1 Antibody | ER-TR9 gallery image 2

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti MouseSIGN-R1 antibody, clone ER-TR9 (MCA2394) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Medium power

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Sign-R1 Antibody | ER-TR9 gallery image 3

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti MouseSIGN-R1 antibody, clone ER-TR9 (MCA2394) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). High power

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Sign-R1 Antibody | ER-TR9 gallery image 4

Published customer image:
Rat anti Mouse SIGN-R1 antibody, clone ER-TR9 (MCA2394) used for the evaluation of SIGN-R1 expression in murine spleen by immunofluorescence.
Image caption:
Propofol exposure leads to reduced numbers of marginal zone macrophages and T cell populations in the spleen, and facilitates dissemination of Lm into the white pulp. Mice were infected with 2 x 104 CFU Lm via intravenous tail vein inoculation in the absence or presence of propofol and sacrificed at 72 hours post-infection. Spleens were harvested, fixed, and antibody-stained for the marginal zone macrophage marker SIGN-RI (red) as well as Lm (green) (a) or for the pan-T cell marker CD3 (green) (c). Images shown are all taken at a 10x magnification and are representative of 3 independent experiments. (b). Quantitation of marginal zone macrophages in the presence or absence of propofol in spleens of Lm-infected animals. Data is an average of 2–4 animals per treatment group, with counts taken from 5 fields per spleen section. Error bars indicate data ± SEM. * p<0.05.

From: Visvabharathy L, Xayarath B, Weinberg G, Shilling RA, Freitag NE (2015) Propofol Increases Host Susceptibility to Microbial Infection by Reducing Subpopulations of Mature Immune Effector Cells at Sites of Infection.
PLoS ONE 10(9): e0138043.

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  • Rat anti Mouse Sign-R1:Alexa Fluor® 488
  • Rat anti Mouse Sign-R1:Alexa Fluor® 647
  • Rat anti Mouse Sign-R1:Alexa Fluor® 488
  • Rat anti Mouse Sign-R1
  • Rat anti Mouse Sign-R1:Low Endotoxin
  • Rat anti Mouse Sign-R1
  • Rat anti Mouse Sign-R1:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ER-TR9
  • Isotype
    IgM
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2394C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA2394
    MCA2394ELC, Fdatasheet pdfdatasheet pdf0.5 mg
    MCA2394EL
    MCA2394A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2394A488
    MCA2394A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2394A647
    MCA2394TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA2394T
    MCA2394A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2394A647T
    MCA2394A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2394A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse SIGN-R1 antibody, clone ER-TR9 recognizes mouse SIGN-R1, a C-type lectin that has been identified as a murine homologue of the human DC-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations.
      Like many C-type lectins, SIGN-R1 functions as a pathogen recognition receptor in vivo and plays an important role in defence against pathogens.

      Rat anti Mouse SIGN-R1, clone ER-TR9, is reported to block the uptake of neutral polysaccharides by macrophages (Kraal et al. 1989).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgM conjugated to Alexa Fluor® 488 - liquid
      Purified IgM conjugated to Alexa Fluor® 647 - liquid
      Purified IgM conjugated to Alexa Fluor® 488 - liquid
      Purified IgM - liquid
      Purified IgM - liquid
      Purified IgM - liquid
      Purified IgM conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Isolated C3H thymic stromal cells
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 0.05 mg/ml
      IgM concentration 0.05 mg/ml
      IgM concentration 0.05 mg/ml
      IgM concentration 1.0 mg/ml
      IgM concentration 1.0 mg/ml
      IgM concentration 1.0 mg/ml
      IgM concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised rats were fused with cells of the Mouse P3-X63-Ag8.563 myeloma cell line
    • Storage
      Store at +4oC or at -20o if preferred.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20o if preferred.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20o if preferred.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20o if preferred.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/251/50
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/251/50
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/251/50
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Sign-R1 Antibody Formats

    Formats Clone Applications Sizes available
    Sign-R1 Antibody : Alexa Fluor® 647 ER-TR9 F 25 Tests/0.25ml | 100 Tests/1ml
    Sign-R1 Antibody : Purified ER-TR9 C, F 25 µg | 0.25 mg
    Sign-R1 Antibody : Alexa Fluor® 488 ER-TR9 F 25 Tests/0.25ml | 100 Tests/1ml
    Sign-R1 Antibody : Low Endotoxin ER-TR9 C, F 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

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