SDF-1 Alpha

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  • Recombinant Mouse SDF-1 Alpha
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP77FNdatasheet pdfdatasheet pdf10 µg
    PMP77
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • SDF-1 alpha, otherwise known as CXCL12a, is a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons.

      SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 100ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant SDF-1 alpha expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      Determined by its ability to chemoattract human peripheral blood monocytes, using a concentration of 50-100 ng/ml.
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml
    • Molecular Weight
      7.9kD (68 amino acid residue homodimer)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      chemokine activity
      external side of plasma membrane
      positive regulation of cell migration
      patterning of blood vessels
      growth factor activity
      germ cell development
      motor axon guidance
      induction of positive chemotaxis
      extracellular space
      germ cell migration
      immune response
      T cell proliferation
      ameboidal cell migration
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional SDF-1 Alpha Formats

    Formats Applications Sizes available
    SDF-1 Alpha : Rec. Protein FN 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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