Rictor Antibody

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Western blot analysis of Jurkat cell lysate (lane 1) and HeLa cell lysate (lane 2) probed with Rabbit anti RICTOR antibody

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  • Rabbit anti Rictor
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2343WBdatasheet pdfdatasheet pdf50 µg
    AHP2343
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti RICTOR antibody recognizes rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR). RICTOR and mTORC2 have been shown to play an essential role in embryonic growth and development, perhaps due to the control that mTORC2 exerts on actin cytoskeleton organization.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      30% Glycerol
      0.5% Bovine Serum Albumin
      0.01% Thiomersal
      5mM EDTA
    • Immunogen
      Synthetic peptide surrounding amino acid 1639 of mouse RICTOR
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      embryo development
      positive regulation of actin filament polymerization
      regulation of Rac GTPase activity
      regulation of protein kinase B signaling cascade
      actin cytoskeleton reorganization
      TORC2 complex
      binding
      positive regulation of peptidyl-tyrosine phosphorylation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti RICTOR antibody recognizes a band of approximately 190kDa in HeLa and Jurkat lysates
    • Instructions For Use

    Additional Rictor Antibody Formats

    Formats Applications Sizes available
    Rictor Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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