Padi2 Antibody | AbD02121

Padi2 Antibody | AbD02121 gallery image 1

Distribution of Padi2 in mouse brain tissue as demonstrated by immunofluorescence staining

  • Padi2 Antibody | AbD02121 thumbnail image 1
  • Human anti Mouse Padi2
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA011C, E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Human anti mouse Padi2 antibody recognizes Peptidylarginine deiminase II also known as Padi2 or mKIAA0994.

      Human anti mouse Padi2 may bind to protein-arginine deiminase type II of others species, due to a high sequence identity in the antigen region. The cross-species identity is: human 98%, rat 98%, chimpanzee 97%, dog 97%, chicken 87%.

      The mKIAA0994 protein catalyzes the deimination of arginine residues of proteins. Protein L-arginine reacts to protein L-citrulline and ammonia. The enzyme requires calcium as cofactor. mKIAA0994 is expressed in various tissues including muscle, uterus, spinal cord, salivary gland and pancreas. It is expressed during the estrous cycle. During diestrus and proestrus cycle, the expression level drops eight fold. The protein belongs to the protein-arginine deiminase family.
    • Intended Use
    • Target Species
    • Product Form
      A lyophilised bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
      Reconstitute with 100 µl distilled water
    • Preparation
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      None present
    • Immunogen
      A fusion protein of a domain of murine mKIAA0994 (amino acid residues 203-389, MW 21 kDa) expressed in E. coli.
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 1.0 mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      6 months from date of reconstitution.
    • GO Terms
      calcium ion binding
      peptidyl-citrulline biosynthetic process from peptidyl-arginine
      protein-arginine deiminase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany, in collaboration with the Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818 JAPAN.

      His-tag is a registered trademark of EMD Biosciences.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen3.6ug/ml
      Western Blotting2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Padi2 Antibody Formats

    Formats Clone Applications Sizes available
    Padi2 Antibody : Purified AbD02121 C, E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      Mouse anti c-MycMCA22001 mgC, E, F*, P, WB*

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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