Numbl Antibody

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Numbl Antibody gallery image 1

Fetal mouse brain cell lysate probed with Goat anti Mouse Numbl (AAM82)

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Numbl Antibody gallery image 2

Formalin fixed, paraffin embedded fetal mouse brain stained with Goat anti Mouse NUMBL (AAM82) following heat mediated antigen retrieval using citrate buffer (pH6.0)

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Numbl Antibody gallery image 3

Formalin fixed, paraffin embedded fetal mouse brain stained with Goat anti Mouse NUMBL (AAM82) following heat mediated antigen retrieval using citrate buffer (pH6.0)

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  • Numbl Antibody thumbnail image 1
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  • Goat anti Mouse Numbl
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM82E, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM82
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Mouse Numbl polyclonal antibody recognizes mouse Numb-like protein (Numbl), a cytoplasmic protein preferentially expressed in developing brain tissue, which plays a key role in neurogenesis, as well as in the regulation of the NF-kappa-B and Notch signaling pathways.

      The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.

      During embryonic neurogenesis, Numbl is essential for the maintenance and cell fate of neural progenitor cells, known as radial glial cells (RGCs), which act as progenitors and migrational guides for neurons, especially vital in cerebral cortex morphogenesis. Studies in mice have linked deletion of the Numbl gene with early embryonic death.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      RatExpected from Sequence
      DogExpected from Sequence
      BovineExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to mouse Numbl was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-PAQPGHVSPTPAT from the internal region of Numbl (NP_035080.2)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      axonogenesis
      adherens junction organization
      cytokine-mediated signaling pathway
      neuroblast division in subventricular zone
      protein metabolic process
      cytoplasm
      lateral ventricle development
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/16000
      Western Blotting0.030.1ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AAM82 detects a band of approximately 75kDa in fetal mouse brain cell lysates.
    • Instructions For Use

    Additional Numbl Antibody Formats

    Formats Applications Sizes available
    Numbl Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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