Notch 2 antibody | HMN2-35
Hamster anti Mouse Notch 2
- Product Type
- Monoclonal Antibody
- Clone
- HMN2-35
- Isotype
- IgG
- Specificity
- Notch 2
Antibody Quality and Performance Guarantee
| Hamster anti Mouse Notch 2 antibody, clone HMN2-35 recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). There is a negative correlation between Notch 2 expression and the grade of human breast cancer. Hamster anti Mouse Notch 2 antibody, clone HMN2-35 cross-reacts with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mouse Notch 2-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| List Price | Your Price | ||||||
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| Description | Hamster (Armenian) IgG Negative Control | ||||||
References for Notch 2 antibody
-
Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
Int Immunol. 20 (6): 763-73. -
Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
Int Immunol. 21 (3): 295-301. -
Gibb, D.R. et al. (2010) ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo.
J Exp Med. 207 (3): 623-35. -
Sakata-Yanagimoto, M. et al. (2011) Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine.
Blood. 117 (1): 128-34.
Further Reading
-
Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
Immunol Rev. 182: 215-27. -
Iso, T. et al. (2003) Notch signaling in vascular development.
Arterioscler Thromb Vasc Biol. 23 (4): 543-53. -
Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
Nat Rev Mol Cell Biol. 7 (9): 678-89. -
Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
Immunity. 29 (5): 691-703.
- RRID
- AB_10708085
- UniProt
- O35516
- Q9QW30
- Entrez Gene
- Notch2
- Notch2
- GO Terms
- GO:0005515 protein binding
- GO:0005509 calcium ion binding
- GO:0016049 cell growth
- GO:0001709 cell fate determination
- GO:0002011 morphogenesis of an epithelial sheet
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005634 nucleus
- GO:0006350 transcription
- View More GO Terms
- GO:0007050 cell cycle arrest
- GO:0007219 Notch signaling pathway
- GO:0007368 determination of left/right symmetry
- GO:0009887 organ morphogenesis
- GO:0008285 negative regulation of cell proliferation
- GO:0009986 cell surface
- GO:0030326 embryonic limb morphogenesis
- GO:0043065 positive regulation of apoptosis
- GO:0045449 regulation of transcription
- GO:0046579 positive regulation of Ras protein signal transduction
- GO:0050793 regulation of developmental process
- GO:0042246 tissue regeneration
MCA5702GA
L1710
MCA5702
L1710
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