Notch 2 Antibody | HMN2-35

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Notch 2 Antibody | HMN2-35 gallery image 1

Mouse Notch 2 transfected cells stained with Hamster anti Mouse Notch 2 (MCA5702)

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  • Notch 2 Antibody | HMN2-35 thumbnail image 1
  • Hamster anti Mouse Notch 2
  • Hamster anti Mouse Notch 2
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  • Product Type
    Monoclonal Antibody
  • Clone
    HMN2-35
  • Isotype
    IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5702GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA5702GA
    MCA5702Fdatasheet pdfdatasheet pdf0.25 mg
    MCA5702
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Hamster anti Mouse Notch 2 antibody, clone HMN2-35 specifically recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.

      The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression.

      Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

      Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer.

      Hamster anti Mouse Notch 2 antibody, clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Notch 2 Antibody Formats

    Formats Clone Applications Sizes available
    Notch 2 Antibody : Purified HMN2-35 F 0.1 mg | 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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