Notch 1 Antibody | mN1A

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Notch 1 Antibody | mN1A gallery image 1

Staining of mouse thymocytes with Mouse anti Mouse NOTCH 1:Alexa Fluor® 488 (MCA2410A488)

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Notch 1 Antibody | mN1A gallery image 2

Staining of mouse thymocytes with Mouse anti Mouse NOTCH-1:Alexa Fluor® 647 (MCA2410A647)

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Notch 1 Antibody | mN1A gallery image 3

Staining of mouse thymocytes with Mouse anti Mouse NOTCH-1:FITC (MCA2410F)

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  • Notch 1 Antibody | mN1A thumbnail image 1
  • Notch 1 Antibody | mN1A thumbnail image 2
  • Notch 1 Antibody | mN1A thumbnail image 3
  • Mouse anti Mouse Notch 1
  • Mouse anti Mouse Notch 1:Alexa Fluor® 647
  • Mouse anti Mouse Notch 1:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    mN1A
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2410GAC, F *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2410GA
    MCA2410FF *datasheet pdfdatasheet pdf0.1 mg
    MCA2410F
    MCA2410A647F *datasheet pdfdatasheet pdf100 Tests/1ml
    MCA2410A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Mouse Notch 1 antibody, clone mN1A specifically recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.

      The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression.

      Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

      Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoeisis, myelopoiesis, and neurogenesis.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/501/200
      Western Blotting
      Flow Cytometry(1)
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (product code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Notch 1 Antibody Formats

    Formats Clone Applications Sizes available
    Notch 1 Antibody : Purified mN1A C, F *, WB 0.1 mg
    Notch 1 Antibody : Alexa Fluor® 647 mN1A F * 100 Tests/1ml
    Notch 1 Antibody : FITC mN1A F * 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

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          Mouse Seroblock FcRBUF041A0.1 mgF
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          Mouse Seroblock FcRBUF041B0.5 mgF
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          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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