NOS-II Antibody

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NOS-II Antibody gallery image 1

Published customer image:
Rabbit anti Human nitric oxide synthase antibody (AHP303) used for the detection of iNOS in stimulated microglial lysates by western blotting.
Image caption:
Inhibition of iNOS and COX-2 expression in LPS-stimulated microglial cells by Af extract. Microglial cells were treated with 100 µg/mL of Af extract, followed by stimulation with LPS for 20 h. Equal amounts of cell lysates were separated by SDS-PAGE and immunoblotted with antibodies to iNOS, COX-2, and β-actin. A. The immunoblot represents one of three different experiments with similar results. B. Data represent the means ± SEM of three independent experiments (n = 3). The levels of each protein were normalized to the levels of β-actin protein.

From: Elmann A, Mordechay S, Erlank H, Telerman A, Rindner M, Ofir R. Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 2011 Oct 21;11:98.

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  • Rabbit anti Inducible Nitric Oxide Synthase
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP303C, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP303
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/500
      Western Blotting1/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NOS-II Antibody Formats

    Formats Applications Sizes available
    NOS-II Antibody : Serum C, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Paraffin

              References

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