Noggin is a secreted polypeptide, originally identified as an antagonist of BMP-4, which binds to and inactivates signal transduction by members of the transforming growth factor-beta (TGF-beta) superfamily, and plays an essential role in cartilage morphogenesis, joint formation and neural tube fusion, as determined by mouse knockout studies. Noggin is implicated in both early and later developmental stages, and can be used as a tool to differentiate human embryonic stem cells into neural precursor cells.
Purified recombinant protein - lyophilised
Reconstitute with 0.2ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Further dilutions can be made in water containing 0.1% BSA.
Purified recombinant Noggin expressed in E.coli
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of Noggin.
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
46.4kD (206 amino acid residue homodimer)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.