NFkB p65 Antibody

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Western blot analysis of Jurkat cell lysate probed with Rabbit anti NFkB p65 antibody

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  • Rabbit anti NFkB p65
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2332IC, WBdatasheet pdfdatasheet pdf50 µg
    AHP2332
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti NFkB p65 antibody recognizes Nuclear factor NF-kappa-B p65 subunit also known as Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 or RelA. NF-kappa-B p105 and p100 subunits are large precursor proteins that are cleaved by the ubiquitin/proteasome pathway into the mature NF-kappa-B p50 and p52 subunits (NF-kappa-B p105 cleavage results in the p50 unit, while cleavage of NF-kappa-B p100 results in the p52 subunit). Rel protein family members interact with p52 and p50 to form transcription factors. In the absence of stimulation an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunocytochemistry1020 ug/ml
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti NFkB p65 antibody primarily recognizes a band of approximately 65 kDa in Jurkat cell lysate
    • Instructions For Use

    Additional NFkB p65 Antibody Formats

    Formats Applications Sizes available
    NFkB p65 Antibody : Purified IC, WB 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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