Mouse anti Mouse NC1.1 antibody, clone IC4 recognizes an alloantigen designated NC1.1 expressed by murine natural cytotoxic cells. (NC1.1 is a 45kDa cell surface antigen).
Natural cytotoxic cells represent a small subset of cells with cytotoxic activity against certain tumor cells in functional assays. Mouse anti Mouse NC1.1 antibody, clone IC4 has been shown to block substantially NC cells activity in vivo and in vitro.
Mouse anti Mouse NC1.1 antibody, clone IC4 recognizes the NC1.1 alloantigen in CBA, C57BL/6, BALB/c and NZB mice, but not in CE or DBA/2 mice.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G
Spleen cells from CBA mice
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunised CE mice were fused with cells of the mouse NS-I myeloma cell line.
Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested wokring dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.