NC1.1 Antibody | IC4

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  • Product Type
    Monoclonal Antibody
  • Clone
    IC4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1763XZF, FN, IP, WBdatasheet pdfdatasheet pdf1 mg
    MCA1763XZ
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Mouse NC1.1 antibody, clone IC4 recognizes an alloantigen designated NC1.1 expressed by murine natural cytotoxic cells. (NC1.1 is a 45kDa cell surface antigen).

      Natural cytotoxic cells represent a small subset of cells with cytotoxic activity against certain tumor cells in functional assays. Mouse anti Mouse NC1.1 antibody, clone IC4 has been shown to block substantially NC cells activity in vivo and in vitro.

      Mouse anti Mouse NC1.1 antibody, clone IC4 recognizes the NC1.1 alloantigen in CBA, C57BL/6, BALB/c and NZB mice, but not in CE or DBA/2 mice.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      None present
    • Immunogen
      Spleen cells from CBA mice
    • Purity
    • Approx. Protein Concentrations
      Isotype concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised CE mice were fused with cells of the mouse NS-I myeloma cell line.
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/40
      Functional Assays
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested wokring dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NC1.1 Antibody Formats

    Formats Clone Applications Sizes available
    NC1.1 Antibody : Preservative Free IC4 F, FN, IP, WB 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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