Ly-6C/CD31 | ERMP20/ERMP12

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Ly-6C/CD31 | ERMP20/ERMP12 gallery image 1

Staining of mouse bone marrow leucocytes with DC054, showing Ly-6C staining on X-axis and CD31 staining on Y-axis

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  • Rat anti Mouse Ly-6C:Alexa Fluor® 488/CD31:RPE-Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Dual Color Reagent
  • Clone
    ERMP20/ERMP12
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    DC054Fdatasheet pdfdatasheet pdf100 Tests
    DC054
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • DC054 is a dual colour reagent containing antibodies which recognise murine Ly-6C and CD31 cell surface antigens. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.

      Antibodies produced by clone ERMP20 recognise murine Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

      Antibodies produced by clone ERMP12 recognise murine CD31, a 140kD cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets, and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Dual Colour combination consisting of Alexa Fluor®488 conjugated and RPE-Alexa Fluor®647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      A488 reagent Rat IgG2a
      RPE-A647 reagent Rat IgG2a
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      Rho protein signal transduction
      cell adhesion
      plasma membrane
      integral to membrane
      anchored to membrane
      cell junction
      regulation of cell migration
      wound healing
      membrane raft
      smooth muscle contractile fiber
      soluble fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.

      The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ly-6C/CD31 Formats

    Formats Clone Applications Sizes available
    Ly-6C/CD31 : Alexa Fluor® 488 / RPE-Alexa FLUOR®647 ERMP20/ERMP12 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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