Ly-6C/CD31 (clone ERMP20/ERMP12) is a dual colour reagent containing antibodies which recognise murine Ly-6C and CD31 cell surface antigens.
Antibodies produced by clone ERMP20 recognise murine Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.
Antibodies produced by clone ERMP12 recognise murine CD31, a 140kD cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets, and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow.
Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.
Dual Colour combination consisting of Alexa Fluor®488 conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitute with 1.0 ml distilled water
Bovine Serum Albumin
Approx. Protein Concentrations
A488 reagent Rat IgG2a RPE reagent Rat IgG2a
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).