anti Mouse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD31:Alexa Fluor® 647
Triple Color Reagent
ERMP20 / 604B5 2E11 / ERMP12
1 Formats Available
Negative Isotype Controls
Anti Mouse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD31:Alexa Fluor® 647 is a triple color reagent containing antibodies which recognize murine Ly-6C, CD115 and CD31 cell surface antigens.
Antibodies produced by clone ERMP20 recognize murine Ly-6C, a 14 kDa differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.
Antibodies produced by clone 604B5 2E11 recognize the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.
Antibodies produced by clone ERMP12 recognize murine CD31, a 140 kDa cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets, and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow.
Triple Color combination consisting of Alexa Fluor® 488, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitute with 1.0 ml distilled water
Bovine Serum Albumin
Approx. Protein Concentrations
Ly-6C A488 reagent: Rat IgG2a CD115 RPE reagent: Rat IgG1 CD31 A647 reagent: Rat IgG2a
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).