Ly-6C/CD115/CD31 | ERMP20 / 604B5 2E11 / ERMP12

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Ly-6C/CD115/CD31 | ERMP20 / 604B5 2E11 / ERMP12 gallery image 1

Staining of mouse bone marrow leucocytes with TC016, showing Ly-6C staining on X-axis and CD115 staining on Y-axis

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Ly-6C/CD115/CD31 | ERMP20 / 604B5 2E11 / ERMP12 gallery image 2

Staining of mouse bone marrow leucocytes with TC016, showing Ly-6C staining on X-axis and CD31 staining on Y-axis

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  • Ly-6C/CD115/CD31 | ERMP20 / 604B5 2E11 / ERMP12 thumbnail image 1
  • Ly-6C/CD115/CD31 | ERMP20 / 604B5 2E11 / ERMP12 thumbnail image 2
  • anti Mse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD31:RPE-Alexa Fluor® 647
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  • Product Type
    Triple Color Reagent
  • Clone
    ERMP20 / 604B5 2E11 / ERMP12
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC016Fdatasheet pdfdatasheet pdf100 Tests
    TC016
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Anti Mouse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD31:RPE-Alexa Fluor® 647 is a triple color reagent containing antibodies which recognize murine Ly-6C, CD115 and CD31 cell surface antigens.

      Antibodies produced by clone ERMP20 recognize murine Ly-6C, a 14 kDa differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

      Antibodies produced by clone 604B5 2E11 recognize the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms.CD115 is expressed by cells of the monocytic lineage and by progenitor cells.

      Antibodies produced by clone ERMP12 recognize murine CD31, a 140 kDa cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets, and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.

      The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ly-6C/CD115/CD31 Formats

    Formats Clone Applications Sizes available
    Ly-6C/CD115/CD31 : 3 Color ERMP20 / 604B5 2E11 / ERMP12 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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