Ly-6C/CD115/CD11b | ERMP20 / 604B5 2E11 / M1/70.15

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  • Ly-6C/CD115/CD11b | ERMP20 / 604B5 2E11 / M1/70.15 thumbnail image 1
  • anti Mse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD11b:RPE-Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Triple Color Reagent
  • Clone
    ERMP20 / 604B5 2E11 / M1/70.15
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC015Fdatasheet pdfdatasheet pdf100 Tests
    TC015
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Anti Mouse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD11b:RPE-Alexa Fluor® 647 is a triple color reagent containing antibodies which recognize murine Ly-6C, CD115 and CD11b cell surface antigens.

      Antibodies produced by clone ERMP20 recognize murine Ly-6C, a 14 kDa differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

      Antibodies produced by clone 604B5 2E11 recognize the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.

      Antibodies produced by clone M1/70.15 recognize the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.

      The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ly-6C/CD115/CD11b Formats

    Formats Clone Applications Sizes available
    Ly-6C/CD115/CD11b : 3 Color ERMP20 / 604B5 2E11 / M1/70.15 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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